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High-resolution structure of a retroviral protease folded as a monomer

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F11%3A00369861" target="_blank" >RIV/61388963:_____/11:00369861 - isvavai.cz</a>

  • Result on the web

    <a href="http://dx.doi.org/10.1107/S0907444911035943" target="_blank" >http://dx.doi.org/10.1107/S0907444911035943</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1107/S0907444911035943" target="_blank" >10.1107/S0907444911035943</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    High-resolution structure of a retroviral protease folded as a monomer

  • Original language description

    Biophysical and NMR studies of M-PMV protease have indicated that in the absence of substrates or inhibitors M-PMV PR should fold into a stable monomer, but the crystal structure of this protein could not be solved by molecular replacement despite countless attempts. The solution was obtained in mr-rosetta using a model constructed by players of the game Foldit. The structure shows a monomeric protein, with the N- and C-termini completely disordered. The flap loop has an unusual curled shape and a different orientation from both the open and closed states known from dimeric retropepsins. This structure provides important information for the design of dimerization inhibitors of retroviral proteases.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

  • CEP classification

    CE - Biochemistry

  • OECD FORD branch

Result continuities

  • Project

    <a href="/en/project/1M0508" target="_blank" >1M0508: New Antivirals and Antineoplastics</a><br>

  • Continuities

    Z - Vyzkumny zamer (s odkazem do CEZ)

Others

  • Publication year

    2011

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Acta Crystallographica Section D-Biological Crystallography

  • ISSN

    0907-4449

  • e-ISSN

  • Volume of the periodical

    D67

  • Issue of the periodical within the volume

    11

  • Country of publishing house

    DK - DENMARK

  • Number of pages

    8

  • Pages from-to

    907-914

  • UT code for WoS article

    000296787400001

  • EID of the result in the Scopus database