The RNA Binding G-patch Domain in Retroviral Protease Is important for infectivity and D-type Morphogenesis of Mason-Pfizer Monkey Virus

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F05%3A00016188" target="_blank" >RIV/60461373:22330/05:00016188 - isvavai.cz</a>

Alternative codes found

RIV/61388963:_____/05:00028127

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

The RNA Binding G-patch Domain in Retroviral Protease Is important for infectivity and D-type Morphogenesis of Mason-Pfizer Monkey Virus

Original language description

Retroviral proteases (PRs) cleave the viral polyprotein precursorsinto functional mature proteins late during particle release and areessential for viral replication. Unlike most retroviruses, -retroviruses,including Mason-Pfizer monkey virus (M-PMV), assembleimmature capsids within the cytoplasm of the cell. The activation of -retroviral proteases must be highly regulated, because processingof the Gag-related polyprotein precursors occurs only after transportof immature capsids to the plasma membrane and budding.Several -retroviral proteases have unique C-terminal extensionsequences, containing a glycine-rich motif (G-patch), which specificallybinds in vitro to single-stranded nucleic acids. In M-PMV PRthe G-patch is removed in vitro as well as in vivo by autoproteolyticprocessing to yield truncated active forms of PR. To investigate therole of the G-patch domain on the virus life cycle, we introducedmutations within the C-terminal domain of protease.Wefound thatthe G-patch domain of

Czech name

G-patch, RNA vazebná doména v retrovirové protease je důležitá pro infektivitu a morfogenesi D typu Mason-Pfizerova opičího viru

Czech description

Proteasy retrovirů štěpí polyproteinové prekursory na funkční zralé proteiny během uvolňování částic a jsou nezbytné pro replikaci retrovirů. M-PMV skládá nezralé virové částice v cytoplasme. Aktivace proteasy musí být vysoce regulovaný proces, protože štěpení prekursorů se děje pouze po transportu nezralých částic k cytoplasmatické membráně a pučení.

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EE - Microbiology, virology

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2005

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Biological chemistry

ISSN

0021-9258

e-ISSN

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Volume of the periodical

280

Issue of the periodical within the volume

51

Country of publishing house

US - UNITED STATES

Number of pages

7

Pages from-to

42106-42112

UT code for WoS article

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EID of the result in the Scopus database

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