Characterization of bacteria in real soil contaminated with persistent halogenated compounds
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F07%3A00017983" target="_blank" >RIV/60461373:22330/07:00017983 - isvavai.cz</a>
Alternative codes found
RIV/61388963:_____/07:00093420
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Characterization of bacteria in real soil contaminated with persistent halogenated compounds
Original language description
Phytoremediation and rhizoremediation have appeared to be efficient ways of removing persistent halogenated compounds, e.g. polychlorinated biphenyls (PCBs), from soil. In our study, we characterized bacteria in a horse radish (Armoracia rusticana) rootzone contaminated with PCBs. Possible PCB-degrading strains were selected by cultivating on mineral medium with biphenyl. According to 16S rRNA gene sequence analysis Arthrobacter sp., Achromobacter sp., Serratia sp. or Sinorhizobium sp. were identified.Further characterization comprised screening for the presence of bphA1 gene (which encodes the first enzyme of the bph operon) and ribosomal protein profiling using MALDI-TOF MS. This method allows much easier, faster and cheaper identification of bacteria, which can be differentiated at the genus, species, and strain level.
Czech name
Charakterizace bakterií v reálné půdě kontaminované perzistentními halogenovanými sloučeninami
Czech description
Fytoremediace a rhizoremediace jsou efektivní způsoby odstraňování perzistentních halogenovaných sloučenin, např. polychlorovaných bifenylů (PCB), z půdy. V naší studii jsme charakterizovali bacterie v kořenové zóně křenu selského. Potenciální degradéřiPCB byly selektovány růstem na minimálním médiu s bifenylem. Sekvenací genů kódujících 16S rRNA byly identifikovány Arthrobacter sp., Achromobacter sp., Serratia sp. nebo Sinorhizobium sp. Další charakterizace zahrnovala screening na přítomnost bphA1 genu a profilaci ribosomálních proteinů pomocí MALDI-TOF.
Classification
Type
D - Article in proceedings
CEP classification
DK - Contamination and decontamination of soil including pesticides
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2007
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Article name in the collection
Book of Proceedings - 4th Symposium on Biosorption and Bioremediation
ISBN
978-80-7080-026-3
ISSN
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e-ISSN
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Number of pages
4
Pages from-to
179-182
Publisher name
VŠCHT Praha
Place of publication
Praha
Event location
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Event date
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Type of event by nationality
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UT code for WoS article
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