Evaluation of Two Food Grade Proliposomes To Encapsulate an Extract of a Commercial Enzyme Preparation by Microfluidization

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F09%3A00022913" target="_blank" >RIV/60461373:22330/09:00022913 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Evaluation of Two Food Grade Proliposomes To Encapsulate an Extract of a Commercial Enzyme Preparation by Microfluidization

Original language description

The entrapment by microfluidization of a commercial enzyme extract (Debitrase DBP20) in liposomes using two food grade proliposome (C and S) preparations was studied. Liposomes obtained at a low microfluidization pressure (4000 psi) were distributed in abimodal population of small (30-40 nm) and large vesicles (300-700 nm). The composition of the proliposome influenced entrapment efficiency and the repartition of the enzyme between the core and the surface of the liposome. More enzyme was associated with the liposomal surface and greater entrapment efficiencies (64%) were obtained for liposomes with the highest negative zeta potential (proliposome C). Increasing microfluidization pressure and increasing the number of passes through the microfluidizerresulted in losses in entrapment efficiency and enzyme activity, due to decreasing liposome size and enzyme denaturation. Entrapment efficiency was not influenced by external pH and enzyme activity was not adversely affected over storage

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

GM - Food industry

OECD FORD branch

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Project

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Continuities

S - Specificky vyzkum na vysokych skolach

Publication year

2009

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Agricultural and Food Chemistry

ISSN

0021-8561

e-ISSN

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Volume of the periodical

57

Issue of the periodical within the volume

8

Country of publishing house

US - UNITED STATES

Number of pages

7

Pages from-to

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UT code for WoS article

000265227600041

EID of the result in the Scopus database

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