Analysis of isoflavonoids in Ficus carica by HPLC-MS and a comparison of extract Cleanup by Immunoaffinity Chromatography (IAC) and Solid Phase Extraction (SPE)

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F10%3A00024198" target="_blank" >RIV/60461373:22330/10:00024198 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Analysis of isoflavonoids in Ficus carica by HPLC-MS and a comparison of extract Cleanup by Immunoaffinity Chromatography (IAC) and Solid Phase Extraction (SPE)
Original language description
Isoflavonoids are plant phenolics known for their multiple pharmacological activities such as estrogenic, antibacterial, antioxidative etc. While legumes are their main source, the presence of isoflavonoids has been reported in about fifty other families. In non-legumes, isoflavonoids may be present in low concentrations, which complicates their detection and identification in complex biological matrixes. Two methods of sample clean-up prior to HPLC-ESI-MS, i.e. SPE (Solid Phase Extraction) and IAC (Immunoaffinity Chromatography), were compared. Conventional C18 reversed-phase sorbent cartridges (Waters Oasis, 30 ?m) were used for SPE. Immunosorbents for IAC were prepared by immobilisation of polyclonal rabbit antibodies against isoflavonoid-BSA (i.e.genistein, biochanin A and daidzein) conjugates on Affi-Gel 10 solid support (Bio-Rad Laboratories). A set of isoflavonoid standards and edible fig?s (Ficus carica) leaves and fruit ethanolic extracts were cleaned-up by two above mentione
Czech name
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Czech description
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Project
<a href="/en/project/GA525%2F09%2F0994" target="_blank" >GA525/09/0994: Chemotaxonomy of isoflavonoids</a><br>
Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year
2010
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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