Development of sorbents for immunoaffinity extraction of isoflavonoids

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F11%3A43892098" target="_blank" >RIV/60461373:22330/11:43892098 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1016/j.phytol.2010.12.005" target="_blank" >http://dx.doi.org/10.1016/j.phytol.2010.12.005</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.phytol.2010.12.005" target="_blank" >10.1016/j.phytol.2010.12.005</a>

Result language
angličtina
Original language name
Development of sorbents for immunoaffinity extraction of isoflavonoids
Original language description
The majority of currently known isoflavonoids has been detected in and isolated from the Leguminosae; however, their presence has also been reported in numerous non-legumes. Detection of these secondary metabolites in non-legumes is impeded by the complex matrix of plant material and low concentration of the analytes. In this study, we present a combination of immunoaffinity chromatography (IAC) and HPLC-ESI-MS as a new tool for the detection of isoflavonoids. Immunoglobulin fractions of polyclonal rabbit antisera against conjugates of bovine serum albumin (BSA) with selected isoflavonoids (i.e. biochanin A, genistein and daidzein) were immobilized on a commercially available Affi-Gel 10 support. Immunosorbents were characterized according to their specificity and binding capacity and used in an off-line combination with HPLC-ESI-MS for a pilot study of isoflavonoid presence in Maclura pomifera and Ficus carica (Moraceae). (C) 2011 Phytochemical Society of Europe. Published by Elsevier
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CB - Analytical chemistry, separation
OECD FORD branch
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Project
<a href="/en/project/GA525%2F09%2F0994" target="_blank" >GA525/09/0994: Chemotaxonomy of isoflavonoids</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year
2011
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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