Classification of protein binders in artist's paints by matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry: an evaluation of principal component analysis (PCA) and soft independent modelling of class analogy (SIMCA)

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F11%3A43892137" target="_blank" >RIV/60461373:22330/11:43892137 - isvavai.cz</a>

Alternative codes found

RIV/00216208:11410/11:10106853

Result on the web

<a href="http://dx.doi.org/10.1002/rcm.5027" target="_blank" >http://dx.doi.org/10.1002/rcm.5027</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/rcm.5027" target="_blank" >10.1002/rcm.5027</a>

Result language

angličtina

Original language name

Classification of protein binders in artist's paints by matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry: an evaluation of principal component analysis (PCA) and soft independent modelling of class analogy (SIMCA)

Original language description

Proteomics techniques are increasingly applied for the identification of protein binders in historical paints. The complex nature of paint samples, with different kinds of pigments mixed into, and degradation by long term exposure to light, humidity andtemperature variations, requires solid analysis and interpretation methods. In this study matrix?assisted laser desorption/ionisation time?of?flight (MALDI?TOF) mass spectra of tryptic?digested paint replicas are subjected to principal component analysis(PCA) and soft independent modelling of class analogy (SIMCA) in order to distinguish proteinaceous binders based on animal glues, egg white, egg yolk and milk casein from each other. The most meaningful peptide peaks for a given protein class will be determined, and if possible, annotated with their corresponding amino acid sequence. The methodology was subsequently applied on egg temperas, as well as on animal glues from different species. In the latter small differences in the MALDI-

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CE - Biochemistry

OECD FORD branch

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Project

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Continuities

S - Specificky vyzkum na vysokych skolach

Publication year

2011

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Rapid Communications in Mass Spectrometry

ISSN

0951-4198

e-ISSN

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Volume of the periodical

25

Issue of the periodical within the volume

11

Country of publishing house

GB - UNITED KINGDOM

Number of pages

10

Pages from-to

1631-1640

UT code for WoS article

000290773800023

EID of the result in the Scopus database

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