Insertion sequences shared by Bordetella species and implications for the biological diagnosis of pertussis syndrome
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F12%3A43894331" target="_blank" >RIV/60461373:22330/12:43894331 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1007/s10096-012-1718-3" target="_blank" >http://dx.doi.org/10.1007/s10096-012-1718-3</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s10096-012-1718-3" target="_blank" >10.1007/s10096-012-1718-3</a>
Alternative languages
Result language
angličtina
Original language name
Insertion sequences shared by Bordetella species and implications for the biological diagnosis of pertussis syndrome
Original language description
The molecular diagnosis of pertussis and parapertussis syndromes is based on the detection of insertion sequences (IS) 481 and 1001, respectively. However, these IS are also detected in the genomes of various Bordetella species, such that they are not specific for either B. pertussis or B. parapertussis. Therefore, we screened the genome of recently circulating isolates of Bordetella species to compare the prevalence of IS481, IS1001 and, also IS1002 with previously published data and to sequence all ISdetected. We also investigated whether the numbers of IS481 and IS1001 copies vary in recently circulating isolates of the different Bordetella species. We used the polymerase chain reaction (PCR) method for screening the genome of circulating isolatesand to prepare the fragments for sequencing. We used Southern blotting and quantitative real-time PCR for quantification of the numbers of IS. We found no significant diversity in the sequences of the IS harboured in the genomes of the Bo
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EE - Microbiology, virology
OECD FORD branch
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Result continuities
Project
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Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2012
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
European Journal Of Clinical Microbiology & Infectious Diseases
ISSN
0934-9723
e-ISSN
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Volume of the periodical
32
Issue of the periodical within the volume
1
Country of publishing house
US - UNITED STATES
Number of pages
7
Pages from-to
89-96
UT code for WoS article
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EID of the result in the Scopus database
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