Characterization of pbt genes conferring increased Pb2+ and Cd2+ tolerance upon Achromobacter xylosoxidans A8

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F13%3A43895479" target="_blank" >RIV/60461373:22330/13:43895479 - isvavai.cz</a>

Result on the web

<a href="http://www.sciencedirect.com/science/article/pii/S0923250813001873" target="_blank" >http://www.sciencedirect.com/science/article/pii/S0923250813001873</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.resmic.2013.10.002" target="_blank" >10.1016/j.resmic.2013.10.002</a>

Result language

angličtina

Original language name

Characterization of pbt genes conferring increased Pb2+ and Cd2+ tolerance upon Achromobacter xylosoxidans A8

Original language description

The cluster of pbtTFYRABC genes is carried by plasmid pA81. Its elimination from Achromobacter xylosoxidans A8 resulted in increased sensitivity towards Pb2+ and Cd2+. Predicted pbtTRABC products share strong similarities with Pb2+ uptake transporter PbrT, transcriptional regulator PbrR, metal efflux P1-ATPases PbrA and CadA, undecaprenyl pyrophosphatase PbrB and its signal peptidase PbrC from Cupriavidus metallidurans CH34. Expression of pbtABC or pbtA in a metal-sensitive Escherichia colt GG48 rendered the strain Pb2+-, Cd2+- and Zn2+-tolerant and caused decreased accumulation of the metal ions. Accumulation of Pb2+, but not of Cd2+ or Zn2+, was promoted in E. coli expressing pbtT. Additional genes of the pbt cluster are pbtF and pbtY, which encode the cation diffusion facilitator (CDF)-like transporter and a putative fatty acid hydroxylase of unlcnown function, respectively. Expression of pbtF did not confer increased metal tolerance upon E. colt GG48, although the protein showed me

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EE - Microbiology, virology

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2013

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Research in Microbiology

ISSN

0923-2508

e-ISSN

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Volume of the periodical

164

Issue of the periodical within the volume

10

Country of publishing house

BE - BELGIUM

Number of pages

10

Pages from-to

1009-1018

UT code for WoS article

000329379400005

EID of the result in the Scopus database

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