Putative P-1B-type ATPase from the bacterium Achromobacter xylosoxidans A8 alters Pb2 /Zn2 /Cd2 -resistance and accumulation in Saccharomyces cerevisiae

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F14%3A43897361" target="_blank" >RIV/60461373:22330/14:43897361 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1016/j.bbamem.2014.01.023" target="_blank" >http://dx.doi.org/10.1016/j.bbamem.2014.01.023</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.bbamem.2014.01.023" target="_blank" >10.1016/j.bbamem.2014.01.023</a>

Result language
angličtina
Original language name
Putative P-1B-type ATPase from the bacterium Achromobacter xylosoxidans A8 alters Pb2 /Zn2 /Cd2 -resistance and accumulation in Saccharomyces cerevisiae
Original language description
PbtA, a putative P-1B-type ATPase from the Gram-negative soil bacterium Achromobacter xylosoxidans A8 responsible for Pb2+/Zn2+/Cd2+-resistance in Escherichia coli, was heterologously expressed in Saccharomyces cerevisiae. When present in Zn2+- and Pb2+/Cd2+-hypersensitive S. cerevisiae strains CM137 and DTY168, respectively, PbtA was able to restore Zn2+- and Pb2+-resistant phenotype. At the same time, the increase of Pb, Zn, and Cd accumulation in yeast was observed. However, Cd2+-tolerance of the pbtA-bearing yeasts dramatically decreased. The PbtA-eGFP fusion protein was localized primarily in the tonoplast and also in the plasma membrane and the perinuclear region corresponding to the endoplasmic reticulum at later growth stages. This indicates that PbtA protein is successfully incorporated into membranes in yeasts. Since PbtA caused a substantial increase of Pb2+/Zn2+-resistance and accumulation in baker's yeast, we propose its further use for the genetic modification of suitable
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CE - Biochemistry
OECD FORD branch
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Project
<a href="/en/project/GA13-28283S" target="_blank" >GA13-28283S: Bridging microbial community ecology and degradation of xenobiotics – the use of metagenomics to investigate microbial degradation potential</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2014
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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