Application of direct analysis in real time ionization--mass spectrometry (DART-MS) in chicken meat metabolomics aiming at the retrospective control of feed fraud

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F13%3A43896687" target="_blank" >RIV/60461373:22330/13:43896687 - isvavai.cz</a>
Result on the web
<a href="http://download.springer.com/static/pdf/626/art%253A10.1007%252Fs11306-013-0495-z.pdf?auth66=1393423614_631f4f81253f3d9df2bf5ad56b22c80e&ext=.pdf" target="_blank" >http://download.springer.com/static/pdf/626/art%253A10.1007%252Fs11306-013-0495-z.pdf?auth66=1393423614_631f4f81253f3d9df2bf5ad56b22c80e&ext=.pdf</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s11306-013-0495-z" target="_blank" >10.1007/s11306-013-0495-z</a>

Result language
angličtina
Original language name
Application of direct analysis in real time ionization--mass spectrometry (DART-MS) in chicken meat metabolomics aiming at the retrospective control of feed fraud
Original language description
Metabolomic fingerprinting enabled by ambient mass spectrometry employing a direct analysis in real time (DART) ion source coupled to a medium?high resolution/accurate mass time-of-flight mass spectrometer (TOFMS) was used as a tool for differentiation between chickens fed by feed that contained 5-8 % (w/w) of chicken bone meal (a banned component) and those representing a reference group, i.e. grown otherwise under the same conditions. In the first step, the sample extraction and DART-TOFMS instrumental conditions were optimized to obtain the broadest possible representation of ionizable compounds occurring in the extracts obtained from chicken muscle and feed on which experimental animals were grown. To this end, a simultaneous (all-in-one) extraction procedure was developed employing water and cyclohexane mixture as the extraction solvents. Under these conditions both polar as well as non-polar metabolites were isolated within a single extraction step. In the next step, metabolomic
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
GM - Food industry
OECD FORD branch
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Project
<a href="/en/project/QI91B306" target="_blank" >QI91B306: Novel strategy for the assessment of the quality and authenticity of food commodities of animal origin in the chain from the farm to the consumer</a><br>
Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2013
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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