Engineered retroviral virus-like particles for receptor targeting

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F14%3A43897528" target="_blank" >RIV/60461373:22330/14:43897528 - isvavai.cz</a>

Result on the web

<a href="http://link.springer.com/article/10.1007%2Fs00705-013-1873-6" target="_blank" >http://link.springer.com/article/10.1007%2Fs00705-013-1873-6</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s00705-013-1873-6" target="_blank" >10.1007/s00705-013-1873-6</a>

Result language

angličtina

Original language name

Engineered retroviral virus-like particles for receptor targeting

Original language description

Retroviral gag proteins, as well as fragments minimally containing the capsid (CA) and nucleocapsid (NC) subunits of Gag, are able to spontaneously assemble into virus-like particles (VLPs). This occurs in mammalian and bacterial cells as well as in in vitro systems. In every circumstance, nucleic acids are incorporated into the forming particles. Here, we took advantage of an in vitro system for the generation of non-enveloped Mason-Pfizer monkey virus (M-PMV) VLPs derived from a self-assembling CA-NCsubunit of Gag. These VLPs were modified through N-terminal extension of CA-NC with short oligopeptides that, after the assembly process, were exposed on the surface of VLPs. The employed N-terminal modifications allowed specific interaction with targetcells expressing prostate-specific membrane antigen. Using this system, we were able to incorporate selected siRNA into forming VLPs and deliver it into the cytosol of target cells. In comparison with other viral vectors designed for targ

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EE - Microbiology, virology

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2014

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Archives of Virology

ISSN

0304-8608

e-ISSN

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Volume of the periodical

159

Issue of the periodical within the volume

4

Country of publishing house

AT - AUSTRIA

Number of pages

12

Pages from-to

677-688

UT code for WoS article

000334055200007

EID of the result in the Scopus database

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