Effects of ampicilin and vancomycin on Staphylococcus aureus biofilms
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F14%3A43898396" target="_blank" >RIV/60461373:22330/14:43898396 - isvavai.cz</a>
Result on the web
<a href="http://www.agriculturejournals.cz/publicFiles/119577.pdf" target="_blank" >http://www.agriculturejournals.cz/publicFiles/119577.pdf</a>
DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Effects of ampicilin and vancomycin on Staphylococcus aureus biofilms
Original language description
The collection of 23 coagulase positive Staphylococcus aureus strains isolated mainly from food in the Czech Republic were tested on the ability to form biofilms in the presence of ampicilin and vancomycin. The antimicrobial sensitivity (16 antibiotics)was determined in all strains by the standard disc diffusion method on Mueller-Hinton agar plates (NCCLS). The resistence to ampicilin was found in 16 strains (69.5%), all strains were susceptible to vancomycin. The formation of biofilm was conducted in96-well, polystyrene microtiter plates COSTAR 3797 in tryptic soy broth (TSB) with 1% glucose for 2424 h at 30oC. Staining with crystal violet (0.1%) was used for biofilm quantification. Ampicillin( 0.5, 2,and 4 mg/l) and vancomycin ( 32, 64 and 128 mg/l) were added: (i) direct addition of the agent to the well at zero time, (ii) after 24 h to washed well, (iii) after 24 h directly to the well with cell suspension. The tested types of ampicilin treatment did not confirm the impact to res
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EE - Microbiology, virology
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2014
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Czech Journal of Food Sciences
ISSN
1212-1800
e-ISSN
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Volume of the periodical
32
Issue of the periodical within the volume
2
Country of publishing house
CZ - CZECH REPUBLIC
Number of pages
8
Pages from-to
137-144
UT code for WoS article
000335282200004
EID of the result in the Scopus database
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