Development of immunochemical method for the detection of new synthetic cannabinoids
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F14%3A43899087" target="_blank" >RIV/60461373:22330/14:43899087 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Development of immunochemical method for the detection of new synthetic cannabinoids
Original language description
Cannabinoids are a class of diverse chemical compounds that act on cannabinoid receptors on cells that repress neurotransmitter release in the brain. The aim of this study was to develop the immunochemical method for the detection of selected synthetic cannabinoids. Two formats of the indirect competitive ELISA were built as combination of conjugate of synthetic cannabinoids with bovine serum albumin (AŠ089-BSA) with polyclonal antibody Ab 197 (with detection limit 9 +- 3 pg/mL), and polyclonal antibodyAb 198 (with detection limit 8 +- 1 pg/mL). The reactivity with structurally or functionally similar substances was tested to verify the specificity of the assembled formats. Antibodies did not interact significantly with the test substances.
Czech name
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Czech description
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Classification
Type
O - Miscellaneous
CEP classification
CB - Analytical chemistry, separation
OECD FORD branch
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Result continuities
Project
<a href="/en/project/VG20122015075" target="_blank" >VG20122015075: New synthetic drugs (NSD) - creation of toxicological database, development and validation of detection methods including fast imunochemical tests, behavioral pharmacology, pharmacokinetics and biotransformation in rats, epidemiology</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2014
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů