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Development of immunochemical method for the boldenone detection

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F14%3A43899086" target="_blank" >RIV/60461373:22330/14:43899086 - isvavai.cz</a>

  • Result on the web

  • DOI - Digital Object Identifier

Alternative languages

  • Result language

    angličtina

  • Original language name

    Development of immunochemical method for the boldenone detection

  • Original language description

    Boldenone and its esters are illegally used (especially by bodybuilders and atlets) to enhance physical condition and increase body mass and muscle strength. The aim of the work was the development of immunochemical method for the detection of boldenone.Two formats of indirect competitive ELISA were built as a combination of immobilization conjugate BOL-3-OVA and antibody PL 131 (with detection limit 17 +- 7 pg/mL) and immobilization conjugate BOL-17-OVA and antibody PL 128 (with detection limit 14 +-7 pg/mL). Analytical abilities of developed ELISAs were verified on series of samples of matrix artificially contaminated with analyte. Recovery of the format with antibody PL 131 achieved 98 % and for variant with PL 128 103 %.

  • Czech name

  • Czech description

Classification

  • Type

    O - Miscellaneous

  • CEP classification

    CB - Analytical chemistry, separation

  • OECD FORD branch

Result continuities

  • Project

    <a href="/en/project/VG20112015045" target="_blank" >VG20112015045: Rapid immunochemical tests for the detection of steroidal anabolics</a><br>

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Others

  • Publication year

    2014

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů