Continuous production of n-butanol by Clostridium pasteurianum DSM 525 using suspended and surface-immobilized cells

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F15%3A43899521" target="_blank" >RIV/60461373:22330/15:43899521 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1016/j.jbiotec.2015.10.008" target="_blank" >http://dx.doi.org/10.1016/j.jbiotec.2015.10.008</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.jbiotec.2015.10.008" target="_blank" >10.1016/j.jbiotec.2015.10.008</a>

Result language
angličtina
Original language name
Continuous production of n-butanol by Clostridium pasteurianum DSM 525 using suspended and surface-immobilized cells
Original language description
For n-butanol production by Clostridium pasteurianum DSM 525, a modified reinforced Clostridium medium was used, where glucose was alternated with glycerol and two kinds of continuous fermentation were tested using suspended and surface immobilized cellson corn stover pieces. A steady state, with butanol productivity of 4.2 g/L h, was reached during the packed-bed continuous fermentation at a dilution rate of 0.44 hMINUS SIGN 1. The average n-butanol concentration, yield and the ratio of n-butanol/liquid by-products were 10.4 g/L, 33 % and 2.5, respectively. Unexpectedly, during continuous fermentation with suspended cells, at a dilution rate of 0.01 hMINUS SIGN 1, steady-state was not achieved and regular oscillations occurred in all measured variables, i.e. concentrations of glycerol, products and the number of cells stained with the fluorescent dyes carboxy fluorescein diacetate and propidium iodide. A possible explanation for oscillatory/steady-state behavior of suspended/surface-
Czech name
—
Czech description
—

Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EI - Biotechnology and bionics
OECD FORD branch
—

Project
<a href="/en/project/TE01020080" target="_blank" >TE01020080: Biorefinery Research Centre of Competence</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2015
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Similar results(10)