Phosphate binding in the active centre of tomato multifunctional nuclease TBN1 and analysis of superhelix formation by the enzyme
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F15%3A43899762" target="_blank" >RIV/60461373:22330/15:43899762 - isvavai.cz</a>
Result on the web
<a href="http://scripts.iucr.org/cgi-bin/paper?S2053230X15018324" target="_blank" >http://scripts.iucr.org/cgi-bin/paper?S2053230X15018324</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1107/S2053230X15018324" target="_blank" >10.1107/S2053230X15018324</a>
Alternative languages
Result language
angličtina
Original language name
Phosphate binding in the active centre of tomato multifunctional nuclease TBN1 and analysis of superhelix formation by the enzyme
Original language description
Tomato multifunctional nuclease TBN1 belongs to the type I nuclease family, which plays an important role in apoptotic processes and cell senescence in plants. The newly solved structure of the N211D mutant is reported. Although the main crystal-packingmotif (the formation of superhelices) is conserved, the details differ among the known structures. A phosphate ion was localized in the active site of the enzyme. The binding of the surface loop to the active centre is stabilized by the phosphate ion, which correlates with the observed aggregation of TBN1 in phosphate buffer. The conserved binding of the surface loop to the active centre suggests biological relevance of the contact in a regulatory function or in the formation of oligomers.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CE - Biochemistry
OECD FORD branch
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Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2015
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Acta Crystallographica Section F-Structural Biology and Crystallization Communications
ISSN
1744-3091
e-ISSN
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Volume of the periodical
71
Issue of the periodical within the volume
1.11.2015
Country of publishing house
US - UNITED STATES
Number of pages
8
Pages from-to
1408-1415
UT code for WoS article
000364557700008
EID of the result in the Scopus database
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