Phosphate binding in the active centre of tomato multifunctional nuclease TBN1 and analysis of superhelix formation by the enzyme

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389013%3A_____%2F15%3A00454706" target="_blank" >RIV/61389013:_____/15:00454706 - isvavai.cz</a>

Alternative codes found

RIV/60077344:_____/15:00456776 RIV/86652036:_____/15:00456776 RIV/60076658:12310/15:43890175 RIV/68407700:21340/15:00309481

Result on the web

<a href="http://dx.doi.org/10.1107/S2053230X15018324" target="_blank" >http://dx.doi.org/10.1107/S2053230X15018324</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1107/S2053230X15018324" target="_blank" >10.1107/S2053230X15018324</a>

Result language

angličtina

Original language name

Phosphate binding in the active centre of tomato multifunctional nuclease TBN1 and analysis of superhelix formation by the enzyme

Original language description

Tomato multifunctional nuclease TBN1 belongs to the type I nuclease family, which plays an important role in apoptotic processes and cell senescence in plants. The newly solved structure of the N211D mutant is reported. Although the main crystal-packing motif (the formation of superhelices) is conserved, the details differ among the known structures. A phosphate ion was localized in the active site of the enzyme. The binding of the surface loop to the active centre is stabilized by the phosphate ion, which correlates with the observed aggregation of TBN1 in phosphate buffer. The conserved binding of the surface loop to the active centre suggests biological relevance of the contact in a regulatory function or in the formation of oligomers.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2015

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS

ISSN

2053-230X

e-ISSN

—

Volume of the periodical

71

Issue of the periodical within the volume

11

Country of publishing house

GB - UNITED KINGDOM

Number of pages

8

Pages from-to

1408-1415

UT code for WoS article

000364557700008

EID of the result in the Scopus database

2-s2.0-84946600896