Antibacterial effect of peptide compound isolated from horseradish (Armoracia rusticana) tissue culture
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F15%3A43900467" target="_blank" >RIV/60461373:22330/15:43900467 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Antibacterial effect of peptide compound isolated from horseradish (Armoracia rusticana) tissue culture
Original language description
Antimicrobial peptides are an abundant group of proteinaceous compounds widely produced in the plant kingdom. Here, we report the purification and partial characterization of a novel antimicrobial peptide compound from horseradish tissue culture (Armoracia rusticana), named Ar-AMP, with bactericidal activity against both Gram-positive and Gram-negative bacteria. The production of antimicrobial peptides was induced with fungi Aspergillus ochraceus. Ar-AMP was isolated by precipitation with amonium sulphate and separated on SPE and RP-HPLC and then characterized by tricine electrophoresis and mass spectrometry. The molecular mass of the Ar-AMP was determined to be 4 828.61 Da. The activity of isolated peptides was tested by agar-well diffusion test and the MIC was estimated with the apparatus Bioscreen C. The Ar-AMP exerted antibacterial activity towards E. coli, P. aeruginosa, S. enteritidis and B. megatherium at tested concentration 100 ?g/ml. The. MIC was estimated 50 ?g/ml for E. col
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CE - Biochemistry
OECD FORD branch
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Result continuities
Project
<a href="/en/project/TA03010877" target="_blank" >TA03010877: Bioremediation and bioactive foils</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2015
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Pharmaceutical, Chemical and Biological Sciences (JPCBS)
ISSN
2348-7658
e-ISSN
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Volume of the periodical
3
Issue of the periodical within the volume
2
Country of publishing house
IN - INDIA
Number of pages
10
Pages from-to
230-239
UT code for WoS article
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EID of the result in the Scopus database
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