Development of liquid chromatography coupled to high resolution tandem mass spectrometry metabolic fingerprinting method for berries and cranberries authentication

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F15%3A43901495" target="_blank" >RIV/60461373:22330/15:43901495 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Development of liquid chromatography coupled to high resolution tandem mass spectrometry metabolic fingerprinting method for berries and cranberries authentication

Original language description

Berry crops belonging to the Vaccinium genus represent a popular source of bioactive compounds, mainly polyphenols. The phytochemicals contained in cranberries have been shown to have a positive effect on cardiovascular and immune systems, some in vitro experiments have also documented anticancer activity. Another well-known effect of these red berries is prevention of urinary tract infections (UTI). Recently, fraud suspicion on cranberries-based products has been reported; Vaccinium vitis-idaea are partly or even totally replaced by cheaper Vaccinium macrocarpon berries. Although PCR methods may provide solution, these are not available in routine laboratories focused on food analysis. On this account, alternative analytical strategies have to be searched. In this study, metabolomic fingerprinting employing instrumental platform consisting of ultra-high performance liquid chromatography coupled to high resolution tandem mass spectrometry (HPLC-HRMS/MS) has to be investigated. For this purpose, polar and non-polar extracts of Vaccinium vitis-idaea and Vaccinium macrocarpon together with their admixtures were prepared and analyzed by HPLC-HRMS/MS. Subsequently, chemometric evaluation was performed to assess the differences between the samples and to identify significant markers, which are present exclusively or more significantly present in one of the fruit species. In parallel, accurate mass of MS and MS/MS spectra, different software packages and different online libraries were tentatively employed for marker identification. More pronounced clustering and significant differences between samples were found for polar extract in both positive and negative ionization modes. Especially, polyphenols (catechin, epicatechin, procianidins), and glycosides (Myricetin 3-glucoside, peonidin pentose) were identified as markers responsible for berries and cranberries differentiation.

Czech name

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Czech description

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Type

O - Miscellaneous

CEP classification

GM - Food industry

OECD FORD branch

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Project

<a href="/en/project/QJ1530272" target="_blank" >QJ1530272: Complex strategies for effective detection of food fraud in the chain production-consumer</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2015

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů