Biomarkers enabling detection of lingonberries (Vaccinum vitis-idaea) replacement by less valued cranberries (Vacinum macrocarpon)

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F15%3A43901496" target="_blank" >RIV/60461373:22330/15:43901496 - isvavai.cz</a>

Result on the web

—

DOI - Digital Object Identifier

—

Result language

angličtina

Original language name

Biomarkers enabling detection of lingonberries (Vaccinum vitis-idaea) replacement by less valued cranberries (Vacinum macrocarpon)

Original language description

In this study, metabolomic fingerprinting employing instrumental platform consisting of ultra-high performance liquid chromatography coupled to high resolution tandem mass spectrometry (HPLC-HRMS/MS) has been investigated. For this purpose, polar (methanolic) and non-polar (hexane : isopropyl alcohol) extracts of 7 authentic varieties of Vaccinium vitis-idaea and 6 authentic varieties of Vaccinium macrocarpon together with their admixtures were prepared and analyzed by U-HPLC-HRMS/MS. Subsequently, chemometric evaluation was performed to assess the differences between the samples and to identify significant markers, which are present exclusively or more significantly in one of the fruit species. In parallel, accurate mass of ions in MS and MS/MS spectra, various software packages and online libraries were employed for tentative marker identification. The PCA (Principal Component Analysis) revealed significant differences between components in prepared extracts of lingonberries and cranberries. Clear clustering was found for both polar and non-polar extracts in positive and negative ionization modes. Especially, polyphenols (catechin, epicatechin, and procianidins) and glycosylated peonidins (peonidin 3-O-glucoside, peonidin pentose) were identified as markers responsible for lingonberries and cranberries differentiation.

Czech name

—

Czech description

—

Type

O - Miscellaneous

CEP classification

GM - Food industry

OECD FORD branch

—

Project

<a href="/en/project/QJ1530272" target="_blank" >QJ1530272: Complex strategies for effective detection of food fraud in the chain production-consumer</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2015

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů