Surface-engineered Saccharomyces cerevisiae displaying a-acetolactate decarboxylase from Acetobacter aceti ssp xylinum

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F16%3A43901672" target="_blank" >RIV/60461373:22330/16:43901672 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1007/s10529-016-2205-1" target="_blank" >http://dx.doi.org/10.1007/s10529-016-2205-1</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s10529-016-2205-1" target="_blank" >10.1007/s10529-016-2205-1</a>

Result language
angličtina
Original language name
Surface-engineered Saccharomyces cerevisiae displaying a-acetolactate decarboxylase from Acetobacter aceti ssp xylinum
Original language description
Objectives To convert a-acetolactate into acetoin by an a-acetolactate decarboxylase (ALDC) to prevent its conversion into diacetyl that gives beer an unfavourable buttery flavour. Results We constructed a whole Saccharomyces cerevisiae cell catalyst with a truncated active ALDC from Acetobacter aceti ssp xylinum attached to the cell wall using the C-terminal anchoring domain of aagglutinin. ALDC variants in which 43 and 69 Nterminal residues were absent performed equally well and had significantly decreased amounts of diacetyl during fermentation. With these cells, the highest concentrations of diacetyl observed during fermentation were 30 % less than those in wort fermented with control yeasts displaying only the anchoring domain and, unlike the control, virtually no diacetyl was present in wort after 7 days of fermentation. Conclusions Since modification of yeasts with ALDC variants did not affect their fermentation performance, the display of a-acetolactate decarboxylase activity is an effective approach to decrease the formation of diacetyl during beer fermentation.
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EI - Biotechnology and bionics
OECD FORD branch
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Project
<a href="/en/project/TE02000177" target="_blank" >TE02000177: Centre for Innovative Use and Strengthening of Competitiveness of Czech Brewery Raw Materials and Products</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2016
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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