Evaluation of viability, metabolic activity and spore quantity in clostridial cultures during ABE fermentation

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F16%3A43901693" target="_blank" >RIV/60461373:22330/16:43901693 - isvavai.cz</a>

Alternative codes found

RIV/60461373:22810/16:43901693

Result on the web

<a href="http://dx.doi.org/10.1093/femsle/fnw031" target="_blank" >http://dx.doi.org/10.1093/femsle/fnw031</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1093/femsle/fnw031" target="_blank" >10.1093/femsle/fnw031</a>

Result language

angličtina

Original language name

Evaluation of viability, metabolic activity and spore quantity in clostridial cultures during ABE fermentation

Original language description

Flow cytometry, in combination with fluorescent staining, was used to evaluate population heterogeneity in acetonebutanol- ethanol fermentation that was carried out with type strain Clostridium beijerinckii NCIMB 8052 and non-type C. pasteurianum NRRL B-598. A combination of propidium iodide (PI) and carboxyfluorescein diacetate (CFDA), PI plus Syto-9 and bis-oxonol (BOX) alone were employed to distinguish between active and damaged cells together with simultaneous detection of spores. These strategies provided valuable information on the physiological state of clostridia. CFDA and PI staining gave the best separation of four distinct subpopulations of enzymatically active cells, doubly stained cells, damaged cells and spores. Proportional representation of cells in particular sub-regions correlated with growth characteristics, fermentation parameters such as substrate consumption and product formation in both species under different cultivation conditions.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EI - Biotechnology and bionics

OECD FORD branch

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Project

<a href="/en/project/TE01020080" target="_blank" >TE01020080: Biorefinery Research Centre of Competence</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2016

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

FEMS MICROBIOLOGY LETTERS

ISSN

0378-1097

e-ISSN

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Volume of the periodical

363

Issue of the periodical within the volume

6

Country of publishing house

GB - UNITED KINGDOM

Number of pages

8

Pages from-to

1-8

UT code for WoS article

000377967000004

EID of the result in the Scopus database

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