Rapid flow cytometric method for viability determination of solventogenic clostridia

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F12%3A43893362" target="_blank" >RIV/60461373:22330/12:43893362 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1007/s12223-012-0131-8" target="_blank" >http://dx.doi.org/10.1007/s12223-012-0131-8</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s12223-012-0131-8" target="_blank" >10.1007/s12223-012-0131-8</a>

Result language

angličtina

Original language name

Rapid flow cytometric method for viability determination of solventogenic clostridia

Original language description

We endeavored to develop a method for viability determination of solventogenic clostridia and to apply it for monitoring acetone-butanol-ethanol (ABE) fermentation. Six fluorescent probes (propidium iodide [PI], ethidium bromide, fluorescein diacetate, carboxyfluorescein diacetate [cFDA], rhodamine 123, bis-(1,3-dibutylbarbituric acid)trimethine oxonol [BOX]) were tested in order to distinguish two subpopulations of live and dead clostridial cells in suspension. Three of them were found to be appropriate (PI, BOX and cFDA) for this purpose. Developed fluorescent staining methods were applied to batch fermentation processes of Clostridium pasteurianum and C. beijerinckii carried out in a laboratory bioreactor under anaerobic conditions. Whereas PI was found to be applicable to both strains, BOX was convenient only for viability determination of C. pasteurianum. Although cFDA can distinguish two cell subpopulations in suspension, it was found to be unsuitable for viability determination

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EI - Biotechnology and bionics

OECD FORD branch

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Project

<a href="/en/project/FR-TI1%2F218" target="_blank" >FR-TI1/218: *THE DESIGN OF THE PRODUCTION OF BUTANOL WITH THE PILOT PLANT TEST VERIFICATION</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2012

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Folia Microbiologica

ISSN

0015-5632

e-ISSN

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Volume of the periodical

57

Issue of the periodical within the volume

4

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

5

Pages from-to

307-311

UT code for WoS article

000305980400013

EID of the result in the Scopus database

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