Effect of Schiff base Cu(II) complexes on signaling pathways in HT-29 cells
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F16%3A43901847" target="_blank" >RIV/60461373:22330/16:43901847 - isvavai.cz</a>
Result on the web
<a href="https://www.spandidos-publications.com/mmr/14/5/4436" target="_blank" >https://www.spandidos-publications.com/mmr/14/5/4436</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3892/mmr.2016.5739" target="_blank" >10.3892/mmr.2016.5739</a>
Alternative languages
Result language
angličtina
Original language name
Effect of Schiff base Cu(II) complexes on signaling pathways in HT-29 cells
Original language description
Schiff base copper (II) complexes are known for their anticancer, antifungal, antiviral and anti-inflammatory activities. The aim of the current study was to investigate biological effects of Schiff base Cu (II) complexes (0.001-100 mu mol/l)-[Cu-2(sal-D, L-glu)(2)(isoquinoline) (2)]center dot 2C(2)H(5)OH (1), [Cu(sal-5-met-L-glu)(H2O)]center dot H2O (2), [Cu(ethanol)(2)(imidazole)(4)][Cu-2(sal-D, L-glu)(2)(imidazole)(2)] (3), [Cu(sal-D, L-glu)(2-methylimidazole)] (4) on the human colon carcinoma cells HT-29, the mouse noncancerous cell line NIH-3T3 and the human noncancerous fibroblast cell line VH10. The results suggested that Cu (II) complexes exhibit cytotoxic effects against the HT-29 cell line, while complexes 3 and 4 were the most effective. Subsequent to 72 h of incubation, apoptosis was observed in the HT-29 cells induced by Cu (II) complexes 1 (0.1, 1, 10 and 50 mu mol/l), 2 (1, 10, 50 and 100 mu mol/l), 3 (0.01, 1, 10 and 50 mu mol/l) and 4 (0.01, 0.1, 1 and 10 mu mol/l). The apoptotic pathways activated by the Cu (II) complexes were identified. The results indicated that complexes 2, 3 and 4 were able to induce the mitochondria-dependent pathway of apoptosis in HT-29 cells, while complex 1 was obsered to activate the extrinsic pathway of apoptosis. The levels of the anti-apoptotic protein Bcl-2 were reduced and those of the pro-apoptotic protein Bax increased following treatment with complexes 2, 3 and 4. Complex 1 had no effect on Bax protein expression. Complexes 2 and 3 induced elevation of cytochrome c (cyt c), while complex 4 induced a time-dependent elevation of cyt c levels. No cyt c was detected in HT-29 cells exposed to complex 1, suggesting that Cu (II) complexes activated the extrinsic pathway of apoptosis. The results from the current study in addition to previous studies suggest that Schiff base Cu(II) complexes have potential as novel anticancer drugs.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
FD - Oncology and haematology
OECD FORD branch
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Result continuities
Project
<a href="/en/project/LO1601" target="_blank" >LO1601: Prague University Analytical Centre II and III - NPU 2015-2020</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2016
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Molecular Medicine Reports
ISSN
1791-2997
e-ISSN
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Volume of the periodical
14
Issue of the periodical within the volume
5
Country of publishing house
GR - GREECE
Number of pages
9
Pages from-to
4436-4444
UT code for WoS article
000387241600055
EID of the result in the Scopus database
2-s2.0-84992388750