Lipidomic analysis of psychrophilic yeasts cultivated at different temperatures
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F16%3A43901986" target="_blank" >RIV/60461373:22330/16:43901986 - isvavai.cz</a>
Alternative codes found
RIV/61388971:_____/16:00463023
Result on the web
<a href="http://dx.doi.org/10.1016/j.bbalip.2016.07.005" target="_blank" >http://dx.doi.org/10.1016/j.bbalip.2016.07.005</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.bbalip.2016.07.005" target="_blank" >10.1016/j.bbalip.2016.07.005</a>
Alternative languages
Result language
angličtina
Original language name
Lipidomic analysis of psychrophilic yeasts cultivated at different temperatures
Original language description
Analysis of polar lipids from eight psychrophilic yeasts (Ctyptococcus victoriae, Cystofilobasidium capitatum, Holtermaniella wattica, Mrakiella aquatica, M. cryoconiti, Rhodotorula lignophila, Kondoa malvinella and Trichosporon aggtelekiense) grown at 4-28 degrees C by hydrophilic interaction liquid chromatography/high resolution electrospray ionization tandem mass spectrometry determined 17 classes of lipids and identified dozens of molecular species of phospholipids including their regioisomers. Most of the yeasts were able to grow over the whole temperature range, reaching the highest biomass at 4 or 10 degrees C. On temperature drop to 4 degrees C, all eight strains showed a significant decrease of MUFA and a simultaneous increase of PUFA such as alpha-linolenic acid, the content of which in the biomass reached up to 20%. We also found alterations in the proportions of individual phospholipids (PI, PE and PC), the PC/PE-ratio decreasing with decreasing temperature. With increasing temperature the content of PoO-PC rose while that of LL-PC decreased, the drop in the content of LL-PC being nearly 100-fold while the content of PoO-PC increased more than twice. A change in temperature brought about changes in molecular species of PC (molecular species PO-PC versus OP-PC) as well as PE, i.e. PO-PE and OP-PE. The phase transition temperature of PO-PC differs from OP-PC by 7 degrees C and the difference between PO-PE and OP-PE is some 10 degrees C; we thus assume that the cell compensates for the adverse temperature effect by changing the fatty acids in the sn-1 and sn-2 positions.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EI - Biotechnology and bionics
OECD FORD branch
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Result continuities
Project
<a href="/en/project/GA14-00227S" target="_blank" >GA14-00227S: Microorganisms as a source of essential fatty acids with focus on extremophile strains</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2016
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS
ISSN
1388-1981
e-ISSN
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Volume of the periodical
1861
Issue of the periodical within the volume
11
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
9
Pages from-to
1634-1642
UT code for WoS article
000385325800005
EID of the result in the Scopus database
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