A 5'P degradation hot spot influences molecular farming of anticancerogenic nuclease TBN1 in tobacco cells

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F16%3A43902001" target="_blank" >RIV/60461373:22330/16:43902001 - isvavai.cz</a>
Alternative codes found
RIV/60077344:_____/16:00464886 RIV/60076658:12310/16:43890774
Result on the web
<a href="http://link.springer.com/article/10.1007/s11240-016-1054-x" target="_blank" >http://link.springer.com/article/10.1007/s11240-016-1054-x</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s11240-016-1054-x" target="_blank" >10.1007/s11240-016-1054-x</a>

Result language
angličtina
Original language name
A 5'P degradation hot spot influences molecular farming of anticancerogenic nuclease TBN1 in tobacco cells
Original language description
Tomato bifunctional nuclease 1 (TBN1) is a polyfunctional protein with anticancerogenic activity originally isolated as an overexpressed protein from viroid-infected tomato. Its molecular farming in plant cells could be a non-expensive source for its biotechnology preparation. So we analysed TBN1 expression in Agrobacterium-infiltrated leaf sectors of Nicotiana benthamiana and in transformed suspension culture of tobacco BY-2 cells. During its transient expression, TBN1 mRNA was strongly degraded within a hot spot localized in the 3' region. This early degradation process was inhibited by PTGS suppressors p19 and p38 resulting in increased TBN1 mRNA and protein yield. In parallel to degradation of TBN1 mRNA, high mRNA levels of two RNA-dependent RNA polymerases were detected in infiltrated leaf sectors, as well as in the transformed tobacco suspension culture BY-2, where low expression of the nuclease was stably maintained. Higher TBN1 mRNA and nuclease activity levels were found during its molecular farming in RDR6-deficient N. benthamiana plants. By fluorescent microscopy of infiltrated and transformed plant cells, the nuclease-GFP fusion protein was shown to be organized in filament-like structures.
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CE - Biochemistry
OECD FORD branch
—

Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2016
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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