Novel PCR-RFLP system based on rpoB gene for differentiation of Cronobacter species
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F17%3A43914607" target="_blank" >RIV/60461373:22330/17:43914607 - isvavai.cz</a>
Result on the web
<a href="https://www.sciencedirect.com/science/article/pii/S0740002015302550?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0740002015302550?via%3Dihub</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.fm.2016.08.004" target="_blank" >10.1016/j.fm.2016.08.004</a>
Alternative languages
Result language
angličtina
Original language name
Novel PCR-RFLP system based on rpoB gene for differentiation of Cronobacter species
Original language description
Bacteria from the genus Cronobacter are opportunistic foodborne pathogens that can cause severe infections. More rapid, cost-effective and reliable methods are still required for the species identification of Cronobacter spp. In this study, we present a novel PCR-RFLP-based method that uses a newly designed pair of primers for the PCR-amplification of a partial rpoB gene sequence (1635 bp). The amplified products of DNA from 80 Cronobacter strains were separately digested with three restriction endonucleases (Csp6I, HinP1I, Mbol). Using the obtained restriction patterns, a PCR-RFLP identification system was created to enable differentiation between all seven currently-known Cronobacter species. The functionality of our method was successfully verified on real food samples. Moreover, the relationships between the Cronobacter species were determined via a phylogenetic tree created from the RFLP patterns.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10606 - Microbiology
Result continuities
Project
<a href="/en/project/GA13-23509S" target="_blank" >GA13-23509S: Proteomic profiling of Cronobacter strains focused on the incidence of virulent proteins</a><br>
Continuities
S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2017
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Food Microbiology
ISSN
0740-0020
e-ISSN
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Volume of the periodical
62
Issue of the periodical within the volume
nemá
Country of publishing house
GB - UNITED KINGDOM
Number of pages
8
Pages from-to
1-8
UT code for WoS article
000390499900001
EID of the result in the Scopus database
2-s2.0-84988462487