Heterocyclic sterol probes for live monitoring of sterol trafficking and lysosomal storage disorders

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F18%3A43915853" target="_blank" >RIV/60461373:22330/18:43915853 - isvavai.cz</a>
Alternative codes found
RIV/68378271:_____/18:00501282 RIV/68378050:_____/18:00501282 RIV/60461373:22340/18:43915853 RIV/60461373:22810/18:43915853
Result on the web
<a href="http://dx.doi.org/10.1038/s41598-018-32776-6" target="_blank" >http://dx.doi.org/10.1038/s41598-018-32776-6</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1038/s41598-018-32776-6" target="_blank" >10.1038/s41598-018-32776-6</a>

Result language
angličtina
Original language name
Heterocyclic sterol probes for live monitoring of sterol trafficking and lysosomal storage disorders
Original language description
The monitoring of intracellular cholesterol homeostasis and trafficking is of great importance because their imbalance leads to many pathologies. Reliable tools for cholesterol detection are in demand. This study presents the design and synthesis of fluorescent probes for cholesterol recognition and demonstrates their selectivity by a variety of methods. The construction of dedicated library of 14 probes was based on heterocyclic (pyridine)-sterol derivatives with various attached fluorophores. The most promising probe, a P1-BODIPY conjugate FP-5, was analysed in detail and showed an intensive labelling of cellular membranes followed by intracellular redistribution into various cholesterol rich organelles and vesicles. FP-5 displayed a stronger signal, with faster kinetics, than the commercial TF-Chol probe. In addition, cells with pharmacologically disrupted cholesterol transport, or with a genetic mutation of cholesterol transporting protein NPC1, exhibited strong and fast FP-5 signal in the endo/lysosomal compartment, co-localizing with filipin staining of cholesterol. Hence, FP-5 has high potential as a new probe for monitoring cholesterol trafficking and its disorders.
Czech name
—
Czech description
—

Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
10401 - Organic chemistry

Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Similar results(10)