Influence of fluorophore and linker length on the localization and trafficking of fluorescent sterol probes

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F20%3A43922074" target="_blank" >RIV/60461373:22330/20:43922074 - isvavai.cz</a>

Alternative codes found

RIV/68378050:_____/20:00538171 RIV/60461373:22340/20:43922074

Result on the web

<a href="https://www.nature.com/articles/s41598-020-78085-9" target="_blank" >https://www.nature.com/articles/s41598-020-78085-9</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1038/s41598-020-78085-9" target="_blank" >10.1038/s41598-020-78085-9</a>

Result language

angličtina

Original language name

Influence of fluorophore and linker length on the localization and trafficking of fluorescent sterol probes

Original language description

Fluorescent sterol probes, comprising a fluorophore connected to a sterol backbone by means of a linker, are promising tools for enabling high-resolution imaging of intracellular cholesterol. In this study, we evaluated how the size of the linker, site of its attachment and nature of the fluorophore, affect the localization and trafficking properties of fluorescent sterol probes. Varying lengths of linker using the same fluorophore affected cell penetration and retention in specific cell compartments. A C-4 linker was confirmed as optimal. Derivatives of heterocyclic sterol precursors attached with identical C-4 linker to different fluorophores at diverse positions also showed significant differences in their binding properties to various intracellular compartments and kinetics of trafficking. Two novel red-emitting probes with good cell permeability, fast intracellular labelling and slightly different distribution displayed very promising characteristics for sterol probes. These probes also strongly labelled endo/lysosomal compartment in cells with pharmacologically disrupted cholesterol transport, or with a genetic mutation of cholesterol transporting protein NPC1, that overlapped with filipin staining of cholesterol. Overall, the present study demonstrates that the physicochemical properties of the fluorophore/linker pairing determine the kinetics of uptake and distribution and subsequently influence the applicability of final probes.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10406 - Analytical chemistry

Project

<a href="/en/project/GA17-02836S" target="_blank" >GA17-02836S: Development of fluorescent probes for determination of content, localization and trafficking of cholesterol within eukaryotic cells</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2020

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Scientific Reports

ISSN

2045-2322

e-ISSN

—

Volume of the periodical

10

Issue of the periodical within the volume

1

Country of publishing house

GB - UNITED KINGDOM

Number of pages

11

Pages from-to

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UT code for WoS article

000603657800019

EID of the result in the Scopus database

2-s2.0-85097631749