Assessing the combined toxicity of the natural toxins, aflatoxin B-1, fumonisin B-1 and microcystin-LR by high content analysis
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F18%3A43916577" target="_blank" >RIV/60461373:22330/18:43916577 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1016/j.fct.2018.09.052" target="_blank" >http://dx.doi.org/10.1016/j.fct.2018.09.052</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.fct.2018.09.052" target="_blank" >10.1016/j.fct.2018.09.052</a>
Alternative languages
Result language
angličtina
Original language name
Assessing the combined toxicity of the natural toxins, aflatoxin B-1, fumonisin B-1 and microcystin-LR by high content analysis
Original language description
As human co-exposure to natural toxins through food and water is inevitable, risk assessments to safeguard health are necessary. Aflatoxin B1 and fumonisin B1, frequent co-contaminants of maize and microcystin-LR, produced in freshwater by cyanobacteria are all naturally occurring potent toxins that threaten human health. Populations in the poorest regions of the world may suffer repeated simultaneous exposure to these contaminants. Using High Content Analysis, multiple cytotoxicity endpoints were measured for the individual toxins and mixtures in various cell lines. Results highlighted that significant cytotoxic effects were observed for aflatoxin B1 in all cell lines while no cytotoxic effects were observed for fumonisin B1 or microcystin-LR. Aflatoxin B1/microcystin-LR was cytotoxic in the order HepG2 > Caco-2 > MDBK. Fumonisin B1/microcystin-LR affected MDBK cells. The ternary mixture was cytotoxic to all cell lines. Most combinations were additive, however antagonism was observed for binary and ternary mixtures in HepG2 and MDBK cell lines at low and high concentrations. Synergy was observed in all cell lines, including at low concentrations. The combination of these natural toxins may pose a significant risk to populations in less developed countries. Furthermore, the study highlights the complexity around trying to regulate for human exposure to multiple contaminants.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30108 - Toxicology
Result continuities
Project
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Continuities
R - Projekt Ramcoveho programu EK
Others
Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
FOOD AND CHEMICAL TOXICOLOGY
ISSN
0278-6915
e-ISSN
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Volume of the periodical
121
Issue of the periodical within the volume
NOV 2018
Country of publishing house
GB - UNITED KINGDOM
Number of pages
14
Pages from-to
527-540
UT code for WoS article
000449242800058
EID of the result in the Scopus database
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