Application of the LAMP method for food pathogens detection

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F19%3A43919566" target="_blank" >RIV/60461373:22330/19:43919566 - isvavai.cz</a>

Alternative codes found

RIV/60461373:22330/19:43919577

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Application of the LAMP method for food pathogens detection

Original language description

The LAMP method (‘loop-mediated isothermal amplification’) is a modern method for rapid and conclusive detection of pathogenic or indicator microorganisms in various food matrices with considerable potential for the future. Its main advantages include speed, robustness, high specificity and selectivity of its reaction. The aim of this work is application of the LAMP method for fast and reliable detection of food pathogens in various food matrices purposefully contaminated under laboratory conditions. The LAMP method was used for the experiment with 10 food matrices based on following methodology. Three samples were prepared from each matrix: one sample was control and two samples were contaminated by Escherichia coli O157:H7 ATCC 43888 and Salmonella enterica subsp. enterica ATCC 51741 (inoculum 102 CFU g-1 food matrix). For the back-detection of pathogens were used so-called the LAMP ‘food panels‘ (the supplier: Amplex Biosystems GmbH, DEU). It was found that in 19 of 20 tested samples, intentionally contaminated by Salmonella enterica subsp. enterica ATCC 51741, was realized successful back detection from 95 % and that in 15 of 20 samples, intentionally contaminated by Escherichia coli O157: H7 ATCC 4388, was realized successful back detection from 75 %. The LAMP method can be used in the food industry at aimed protection of health safety of food raw materials and products.

Czech name

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Czech description

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Type

O - Miscellaneous

CEP classification

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OECD FORD branch

10606 - Microbiology

Project

<a href="/en/project/QK1710156" target="_blank" >QK1710156: New approaches and methods of analysis to ensure the quality, safety and health wholesomeness of cheeses, the optimization of their production and the streamline of hygiene and sanitation together with the lowering of environmental load by waste water.</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2019

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů