AUTHENTICATION OF OPIUM POPPY (PAPAVER SOMNIFERUM L.) USING DNA ANALYSIS

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F19%3A43919821" target="_blank" >RIV/60461373:22330/19:43919821 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
AUTHENTICATION OF OPIUM POPPY (PAPAVER SOMNIFERUM L.) USING DNA ANALYSIS
Original language description
Opium poppy (Papaver somniferum L.) is an important oilseed using in food and pharmaceutical industry. In the Czech Republic, cultivation of P. somniferum L. has a long tradition. Czech poppy seeds have good nutritional and sensory quality and low levels of opiates. Therefore, poppy seeds are an important ingredient in food item not only in the Czech Republic but also in many countries of Central-Eastern Europe. They are widely used for the preparation of pastry, such as cakes or buns. Recently, there have been several cases of poppy seed falsification. Food poppy seeds were mixed with cheaper seeds from pharmaceutical varieties that contain a higher amount of opium alkaloids and have worse sensory properties. In this work, we tested the possibility to differentiate P. somniferum L. species and varieties by DNA analysis of genes important for the biosynthetic pathway of opium alkaloids, such as codeinone reductase (COR) or reticuline epimerase (REPI). For DNA analysis, we used polymerase chain reaction (PCR). The selected sequence of the COR gene allows distinguishing P. somniferum L. from other tested poppy species. REPI gene seems to be suitable for the differentiation of poppy varieties with low- and high-alkaloid content.
Czech name
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Czech description
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Project
<a href="/en/project/QK1720263" target="_blank" >QK1720263: Diagnostic methods for laboratory control of Papaver L. authenticity</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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