USE OF DNA ANALYSIS FOR THE STUDY OF MEAT AND FISH FRAUD

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F19%3A43919822" target="_blank" >RIV/60461373:22330/19:43919822 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

USE OF DNA ANALYSIS FOR THE STUDY OF MEAT AND FISH FRAUD

Original language description

Food fraud is the current and serious problem on a global scale. Fish, meat and their products are one of the most expensive foods and therefore fall into the category of the adulterated commodities. One common way of deceiving customers is to replace high-quality meat with a less valuable and/or incorrect labeling of the product. Currently, many methods for determining species origin of meat exist. These include molecular-biological methods using DNA analysis, which allow very precise identification of the animal species used in food production.In this work, the polymerase chain reaction (PCR) was successfully used for identification of fish from the Scombridae family (mackerel, tuna, bonito), meat (cattle, pig, horse, chicken, duck, turkey) and products purchased in commercial sources in the Czech Republic. The multiplex PCR method was used for the qualitative determination of meat content in products. The meat of cattle, pigs, poultry, and horses was detected using primers complementary to the gene coding for cytochrome b (mitochondrial DNA). Primers specifically amplifying interleukin II (nuclear DNA) were used to differentiate poultry; species identification of fish was performed by analyzing the sequence of the parvalbumin gene (nuclear DNA). Quantitative multiplex PCR with real-time fluorescence detection (mqPCR) was used to quantify DNA of selected meat species (beef, pork, and chicken) in meat products. In the mqPCR reaction, primers were used in combination with appropriate hydrolysis probes complementary to the DNA encoding the porcine beta-actin, beef cyclic-GMP-phosphodiesterase genes, chicken interleukin II and the gene encoding myostatin, which is a universal marker for mammals and poultry. In some cases, discrepancies between the results of DNA analysis and content of the sample declared by producer were determined.

Czech name

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Czech description

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Type

O - Miscellaneous

CEP classification

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OECD FORD branch

40101 - Agriculture

Project

<a href="/en/project/QK1910231" target="_blank" >QK1910231: New approaches for the proof of fish meat adulteration using genomic DNA</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2019

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů