Quality of colostrum as estimated by different methods

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F19%3A43919933" target="_blank" >RIV/60461373:22330/19:43919933 - isvavai.cz</a>

Result on the web

<a href="https://www.icar.org/wp-content/uploads/2019/12/ICAR-Technical-Series-24-Prague-2019-Proceedings.pdf" target="_blank" >https://www.icar.org/wp-content/uploads/2019/12/ICAR-Technical-Series-24-Prague-2019-Proceedings.pdf</a>

DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Quality of colostrum as estimated by different methods

Original language description

Colostrum is initial secretion produced in mammary glands of mammals following parturition. Colostrum is rich in immunoglobulins, lactoferrin, growth factors and many other biologically active substances. Quality of colostrum is important for new-borns, which have immature immune system, moreover colostrum is also widely used as food supplement. The main quality marker of colostrum is concentration of immunoglobulins, in the broad sense content of proteins. The standard method for estimation of immunoglobulin IgG1 is radial immunodiffusion (RID), but this method is lenghty and expensive. Thirty samples of spray dried or lyophilized cow colostrum were analysed by several methods, both rapid screening methods and more precise methods. Measurement of refraction index is rapid and simple method, but correlation with RID is quite week (below 0.8). Three chromatographic methods were tested: size exclusion chromatography (SEC), affinity chromatography (AC) and reverse phase chromatography. Good results were obtained from SEC (R2=0.95), but column is very expensive and it has short lifetime. Similar results gave affinity chromatography, its advantage is short analysis time (8 min). Spectrophotometric methods (Bradford and UV spectroscopy) are not demanding for instrumentation, but the sample preparation is quite complex.

Czech name

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Czech description

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Type

D - Article in proceedings

CEP classification

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OECD FORD branch

40201 - Animal and dairy science; (Animal biotechnology to be 4.4)

Project

<a href="/en/project/QJ1210376" target="_blank" >QJ1210376: Colostrum as a source of new primary products in food and food supplements characterized by improved dietary properties and high content of natural biologically active substances.</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2019

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection

Proceedings of the ICAR Conference. ICAR Technical Series No 24 - New traits and adding new value to the recording and ID services in the animal production

ISBN

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ISSN

1563-2504

e-ISSN

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Number of pages

5

Pages from-to

155-159

Publisher name

ICAR

Place of publication

Rome

Event location

Prague

Event date

Jun 17, 2019

Type of event by nationality

WRD - Celosvětová akce

UT code for WoS article

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