Distribution of extracellular DNA in Listeria monocytogenes biofilm

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F19%3A43920130" target="_blank" >RIV/60461373:22330/19:43920130 - isvavai.cz</a>
Result on the web
<a href="https://www.agriculturejournals.cz/publicFiles/9_2019-CJFS.pdf" target="_blank" >https://www.agriculturejournals.cz/publicFiles/9_2019-CJFS.pdf</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.17221/9/2019-CJFS" target="_blank" >10.17221/9/2019-CJFS</a>

Result language
angličtina
Original language name
Distribution of extracellular DNA in Listeria monocytogenes biofilm
Original language description
Extracellular DNA (eDNA) is an abundant matrix component that protects biofilm from environmental stress, facilitate horizontal gene transfer, and serve as a source of nutrients. eDNA is also found in Listeria monocytogenes biofilm, but it is unknown to which extent its importance as a matrix component varies in terms of phylogenetic relatedness. This study aims to determine if these variations exist. Biofilm forming capacity of ten L. monocytogenes strains of different phylogenetic lineages and serotypes was examined using crystal violet assay at 37 °C and 22 °C. eDNA content was evaluated fluorometrically at 37 °C and at 22 °C, then the 3D structure of biofilm was studied by confocal laser scanning microscopy (CLSM). Biofilm forming capacity differed significantly between the culturing conditions and was higher at 37 °C than at ambient temperature. eDNA signal distribution was found to be influenced by strain and lineage. CLSM images revealed information about spatial distribution in the biofilm. The information about the eDNA spatial organisation in the biofilm contributes to the understanding of the role of eDNA in a biofilm formation.
Czech name
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Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
10606 - Microbiology

Project
<a href="/en/project/GA17-15936S" target="_blank" >GA17-15936S: Interaction of nanoparticles modified by natural compounds with biofilms of pathogenic microorganisms</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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