Contribution to determination of extracellular DNA origin in the biofilm matrix

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F21%3A43923683" target="_blank" >RIV/60461373:22330/21:43923683 - isvavai.cz</a>

Result on the web

<a href="https://onlinelibrary.wiley.com/doi/epdf/10.1002/jobm.202100090" target="_blank" >https://onlinelibrary.wiley.com/doi/epdf/10.1002/jobm.202100090</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/jobm.202100090" target="_blank" >10.1002/jobm.202100090</a>

Result language

angličtina

Original language name

Contribution to determination of extracellular DNA origin in the biofilm matrix

Original language description

This study is focused on the analysis of extracellular DNA (eDNA) from a biofilm matrix formed by Staphylococcus aureus, Listeria monocytogenes, and Salmonella enterica. The presence of eDNA in the biofilm of all the studied strains was confirmed by confocal laser scanning microscopy using fluorescent dyes with high affinity to nucleic acid. The protocol for eDNA isolation from the biofilm matrix was established, and subsequent characterization of the eDNA was performed. The purified eDNA obtained from the biofilm matrix of all three microorganisms was compared to the genomic DNA (gDNA) isolated from relevant planktonic grown cells. The process of eDNA isolation consisted of biofilm cultivation, its collection, sonication, membrane filtration, dialysis, lyophilisation, and extraction of DNA separated from the biofilm matrix with cetyltrimethylammonium bromide. An amplified fragment length polymorphism (AFLP) was used for comparing eDNA and gDNA. AFLP profiles showed a significant similarity between eDNA and gDNA at the strain level. The highest similarity, with a profile concordance rate of 94.7% per strain, was observed for S. aureus, L. monocytogenes, and S. enterica exhibited lower profiles similarity (78% and 60%, respectively). The obtained results support the hypothesis that the eDNA of studied bacterial species has its origin in the gDNA. © 2021 Wiley-VCH GmbH

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10606 - Microbiology

Project

<a href="/en/project/GA17-15936S" target="_blank" >GA17-15936S: Interaction of nanoparticles modified by natural compounds with biofilms of pathogenic microorganisms</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2021

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Basic Microbiology

ISSN

1521-4028

e-ISSN

—

Volume of the periodical

61

Issue of the periodical within the volume

7

Country of publishing house

DE - GERMANY

Number of pages

10

Pages from-to

652-661

UT code for WoS article

000651038600001

EID of the result in the Scopus database

2-s2.0-85105783136