PURIFICATION OF WATER-SOLUBLE BIOACTIVE POLYSACCHARIDES FROM BIOMASS OF HETEROTROPHIC MUTANT ALGA CHLORELLA VULGARIS G11

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F19%3A43920813" target="_blank" >RIV/60461373:22330/19:43920813 - isvavai.cz</a>
Alternative codes found
RIV/61388971:_____/19:00534790
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
PURIFICATION OF WATER-SOLUBLE BIOACTIVE POLYSACCHARIDES FROM BIOMASS OF HETEROTROPHIC MUTANT ALGA CHLORELLA VULGARIS G11
Original language description
Water extract obtained from biomass of mutant alga Chlorella vulgaris G11 was deproteinized and then purified with anion-exchange chromatography (AEC) on DEAE Sepharose Fast Flow column and gel filtration chromatography on Sepaphadex G-75 superfine column. Two high MW (>1000 kDa and 98.3 kDa) polysaccharides (CPS1 and CPS3) were eluted with water and 0.5M NaCl on DEAE column and heteropolysaccharide with MW of 15 kDa (CPS2) was also eluted with water on DEAE. The former fractions were identified as branched rhamnan; the latter one as branched xylogalactofuranan of unusual structure. CPS1 showed properties of an unstable polymer: according to GPC analysis and by using light scattering and RI detectors it was found that one fraction of polymers (260-800 kDa) has self-assembling properties in aqueous solution and MW of this fraction decreased during some time. On the other hand, polymer with initially larger MW (>1000 kDa) became even bigger. CPS3 was eluted with 0.3-0.5 M of NaCl on DEAE Sepharose FF column and have MW of 98.3 kDa. Cell toxicity of the products was tested in vitro on cell cultures for different concentrations.
Czech name
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Czech description
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Project
<a href="/en/project/FV10155" target="_blank" >FV10155: Algal Biotechnology for Food - preparation of proteins and carotenes by heterotrophic cultivation of chlorophyl-deficient clones of microalgae</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection
PROCEEDINGS OF THE 15TH INTERNATIONAL CONFERENCE ON POLYSACCHARIDES-GLYCOSCIENCE
ISBN
978-80-88307-04-4
ISSN
2336-6796
e-ISSN
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Number of pages
6
Pages from-to
158-163
Publisher name
Česká společnost chemická
Place of publication
Praha
Event location
Praha
Event date
Nov 13, 2019
Type of event by nationality
WRD - Celosvětová akce
UT code for WoS article
000544671400037

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