Isolation of pathogenesis-related proteins from TMV-Infected tobacco and their influence on infectivity of TMV

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F05%3A00028420" target="_blank" >RIV/61389030:_____/05:00028420 - isvavai.cz</a>

Result on the web

—

DOI - Digital Object Identifier

—

Result language

angličtina

Original language name

Isolation of pathogenesis-related proteins from TMV-Infected tobacco and their influence on infectivity of TMV

Original language description

The composition of pathogenesis-related proteins (PR-proteins) and inhibitory influence on TMV multiplication were studied. The ICF PR-proteins of infected plants were separated after solubilisation by decreasing gradient of ammonium sulphate, the cell PR-proteins were separated after acidic homogenisation of leaf tissues. The ICF and cell PR-proteins were further purified by ion exchange chromatography on DEAE cellulose. Using discontinuous non-denaturating polyacrylamide gel electrophoresis of DEAE cellulose fractions the PR-proteins were detected. Molecular weights were estimated by SDS-PAGE. The ICF and cell proteins of infected leaves included PR-proteins of the molecular weights 15?16 kDa (Group 1), 27?28 kDa (Group 3: chitinases) and 36?40 kDa (Group 2a: ?-1,3-glucanases). Fractions with different PR-proteins were tested for their effect on infectivity of TMV. Particularly the PR3 and PR2a proteins seem to decrease the infectivity of TMV.

Czech name

Izolace ?pathogenesis-related? proteinů z tabáku infikovaného virem TMV a jejich vliv na infektivitu viru TMV

Czech description

Z listů hypersenzitivního kultivaru tabáku Xanthi-nc infikovaného virem Tobacco mosaic virus (TMV) byly izolovány ?pathogenesis-related? proteiny (PR-proteiny) jak z intercelulárních prostorů (ICF), tak ze zbývajících pletiv, a byl studován jejich vliv na multiplikaci TMV. PR-proteiny ICF byly purifikovány solubilizací klesajícím gradientem síranu amonného, buněčné proteiny kyselou homogenizací zbývajících listových pletiv. ICF a buněčné PR-proteiny byly dále purifikovány iontoměničovou chromatografií na DEAE celulóze. Jednotlivé PR-proteiny byly detekovány diskontinuální nedenaturující elektroforézou na polyakrylamidovém gelu (PAGE) a jejich molekulové hmotnosti byly určeny pomocí SDS-PAGE. Buněčné i ICF proteiny obsahovaly stejné PR-proteiny o molekulových hmotnostech 15?16 kDa (skupina 1), 27?28 kDa (skupina 3: chitinasy) a 36?40 kDa (skupina 2a: ?-1,3-glukanasy). Infektivitu TMV významně inhibovaly PR-proteiny skupin PR3 a PR2a.

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CE - Biochemistry

OECD FORD branch

—

Project

<a href="/en/project/GA522%2F02%2F0708" target="_blank" >GA522/02/0708: The role of chloroplasts in mechanisms involved in replication and translation of viral-RNA in connection with host resistance</a><br>

Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2005

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Plant Protection Science

ISSN

1212-2580

e-ISSN

—

Volume of the periodical

41

Issue of the periodical within the volume

-

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

6

Pages from-to

52-57

UT code for WoS article

—

EID of the result in the Scopus database

—