Authentication of meat and meat products using triacylglycerols profiling and by DNA analysis
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F20%3A43921032" target="_blank" >RIV/60461373:22330/20:43921032 - isvavai.cz</a>
Result on the web
<a href="https://www.mdpi.com/2304-8158/9/9/1269/htm" target="_blank" >https://www.mdpi.com/2304-8158/9/9/1269/htm</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3390/foods9091269" target="_blank" >10.3390/foods9091269</a>
Alternative languages
Result language
angličtina
Original language name
Authentication of meat and meat products using triacylglycerols profiling and by DNA analysis
Original language description
Two alternative, complementary analytical strategies were successfully used to identify the most common meat species—beef, pork and chicken—in meat products. The first innovative high-throughput approach was based on triacylglycerols fingerprinting by direct analysis in real time coupled with high-resolution mass spectrometry (DART–HRMS). The second was the classic commonly used DNA analysis based on the use of nuclear or mitochondrial DNA in multiplex polymerase chain reaction (mPCR). The DART–HRMS method represents a rapid, high throughput screening method and was shown to have a good potential for the authentication of meat products. Nevertheless, it should be noted that due to a limited number of samples in this pilot study, we present here a proof of concept. More samples must be analyzed by DART–HRMS to build a robust classification model applicable for reliable authentication. To verify the DART–HRMS results, all samples were analyzed by PCRs. Good compliance in samples classification was documented. In routine practice under these conditions, screening based on DART–HRMS could be used for identification of suspect samples, which could be then examined and validated by accurate PCRs. In this way, saving of both labor and cost could be achieved. In the final phase, commercially available meat products from the Czech market were tested using this new strategy. Canned meats—typical Czech sausages and luncheon meats, all with declared content of beef, pork and chicken meat—were used. Compliance with the label declaration was confirmed and no adulteration was found.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10406 - Analytical chemistry
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Foods
ISSN
2304-8158
e-ISSN
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Volume of the periodical
9
Issue of the periodical within the volume
9
Country of publishing house
CH - SWITZERLAND
Number of pages
19
Pages from-to
1269
UT code for WoS article
000581509100001
EID of the result in the Scopus database
2-s2.0-85091416165