Occurrence of ochratoxin A and its stereoisomeric degradation product in various types of coffee available in the Czech market

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F20%3A43921033" target="_blank" >RIV/60461373:22330/20:43921033 - isvavai.cz</a>

Result on the web

<a href="https://www.wageningenacademic.com/doi/10.3920/WMJ2019.2507" target="_blank" >https://www.wageningenacademic.com/doi/10.3920/WMJ2019.2507</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3920/WMJ2019.2507" target="_blank" >10.3920/WMJ2019.2507</a>

Result language

angličtina

Original language name

Occurrence of ochratoxin A and its stereoisomeric degradation product in various types of coffee available in the Czech market

Original language description

Ochratoxin A (OTA) belongs among the most frequently occurring mycotoxins in coffee. In order to investigate its contamination levels in products currently available in the market, a broad set of coffee samples (103 in total) collected between 2016 and 2018 in the Czech Republic was investigated. Aqueous-methanolic extracts purified by using immunoaffinity columns were analysed by ultra-performance liquid chromatography coupled with tandem mass spectrometry (U-HPLC-MS/MS). The undertaken study revealed a relatively low OTA contamination of roasted coffee (in the range 0.2-2.5 µg/kg with the mean concentration of 0.6 µg/kg, and 71% of positive samples). The roasted coffee samples did not exceed the maximum limit of 5 µg/kg set by 1881/2006/EC. With regard to instant coffee samples, OTA concentrations were considerably higher. All the samples were positive, with a mean concentration of 2.9 µg/kg (ranging from 0.6 to 12.8 µg/kg, with 100% of positive samples). One of the analysed samples of instant coffee even exceeded the maximum limit of 10 µg/kg (1881/2006/EC). The study further revealed a relatively high incidence of 14-(R)-OTA, stereoisomer of OTA (14-(S)-OTA), originating as its main degradation product. Its identity was confirmed by high resolution mass spectrometry (HRMS/MS). Most of the samples positive for OTA were also positive for this diastereoisomer, with signal intensities of approx. one-third to one half of the signal of 14-(S)-OTA.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

21101 - Food and beverages

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2020

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

WORLD MYCOTOXIN JOURNAL

ISSN

1875-0710

e-ISSN

1875-0796

Volume of the periodical

13

Issue of the periodical within the volume

1

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

11

Pages from-to

97-107

UT code for WoS article

000512312600009

EID of the result in the Scopus database

2-s2.0-85079236175