Changes in efflux pump activity of Clostridium beijerinckii throughout ABE fermentation

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F21%3A43922715" target="_blank" >RIV/60461373:22330/21:43922715 - isvavai.cz</a>

Alternative codes found

RIV/00216305:26220/21:PU138381

Result on the web

<a href="https://link.springer.com/article/10.1007/s00253-020-11072-2" target="_blank" >https://link.springer.com/article/10.1007/s00253-020-11072-2</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s00253-020-11072-2" target="_blank" >10.1007/s00253-020-11072-2</a>

Result language

angličtina

Original language name

Changes in efflux pump activity of Clostridium beijerinckii throughout ABE fermentation

Original language description

Abstract: Pumping toxic substances through a cytoplasmic membrane by protein transporters known as efflux pumps represents one bacterial mechanism involved in the stress response to the presence of toxic compounds. The active efflux might also take part in exporting low-molecular-weight alcohols produced by intrinsic cell metabolism; in the case of solventogenic clostridia, predominantly acetone, butanol and ethanol (ABE). However, little is known about this active efflux, even though some evidence exists that membrane pumps might be involved in solvent tolerance. In this study, we investigated changes in overall active efflux during ABE fermentation, employing a flow cytometric protocol adjusted for Clostridia and using ethidium bromide (EB) as a fluorescence marker for quantification of direct efflux. A fluctuation in efflux during the course of standard ABE fermentation was observed, with a maximum reached during late acidogenesis, a high efflux rate during early and mid-solventogenesis and an apparent decrease in EB efflux rate in late solventogenesis. The fluctuation in efflux activity was in accordance with transcriptomic data obtained for various membrane exporters in a former study. Surprisingly, under altered cultivation conditions, when solvent production was attenuated, and extended acidogenesis was promoted, stable low efflux activity was reached after an initial peak that appeared in the stage comparable to standard ABE fermentation. This study confirmed that efflux pump activity is not constant during ABE fermentation and suggests that undisturbed solvent production might be a trigger for activation of pumps involved in solvent efflux. Key points: • Flow cytometric assay for efflux quantification in Clostridia was established. • Efflux rate peaked in late acidogenesis and in early solventogenesis. • Impaired solventogenesis led to an overall decrease in efflux. © 2021, The Author(s), under exclusive licence to Springer-Verlag GmbH, DE part of Springer Nature.

Czech name

—

Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

20901 - Industrial biotechnology

Project

<a href="/en/project/GA17-00551S" target="_blank" >GA17-00551S: Relationship between butanol efflux and butanol tolerance of Clostridia</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2021

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Applied Microbiology and Biotechnology

ISSN

0175-7598

e-ISSN

—

Volume of the periodical

105

Issue of the periodical within the volume

2

Country of publishing house

US - UNITED STATES

Number of pages

13

Pages from-to

877-889

UT code for WoS article

000605545300014

EID of the result in the Scopus database

2-s2.0-85099021613