Deeper below the surface-transcriptional changes in selected genes of Clostridium beijerinckii in response to butanol shock
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F21%3A43921030" target="_blank" >RIV/60461373:22330/21:43921030 - isvavai.cz</a>
Result on the web
<a href="https://onlinelibrary.wiley.com/doi/pdfdirect/10.1002/mbo3.1146" target="_blank" >https://onlinelibrary.wiley.com/doi/pdfdirect/10.1002/mbo3.1146</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/mbo3.1146" target="_blank" >10.1002/mbo3.1146</a>
Alternative languages
Result language
angličtina
Original language name
Deeper below the surface-transcriptional changes in selected genes of Clostridium beijerinckii in response to butanol shock
Original language description
The main bottleneck in the return of industrial butanol production from renewable feedstock through acetone-butanol-ethanol (ABE) fermentation by clostridia, such as Clostridium beijerinckii, is the low final butanol concentration. The problem is caused by the high toxicity of butanol to the production cells, and therefore, understanding the mechanisms by which clostridia react to butanol shock is of key importance. Detailed analyses of transcriptome data that were obtained after butanol shock and their comparison with data from standard ABE fermentation have resulted in new findings, while confirmed expected population responses. Although butanol shock resulted in upregulation of heat shock protein genes, their regulation is different than was assumed based on standard ABE fermentation transcriptome data. While glucose uptake, glycolysis, and acidogenesis genes were downregulated after butanol shock, solventogenesis genes were upregulated. Cyclopropanation of fatty acids and formation of plasmalogens seem to be significant processes involved in cell membrane stabilization in the presence of butanol. Surprisingly, one of the three identified Agr quorum-sensing system genes was upregulated. Upregulation of several putative butanol efflux pumps was described after butanol addition and a large putative polyketide gene cluster was found, the transcription of which seemed to depend on the concentration of butanol.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10606 - Microbiology
Result continuities
Project
<a href="/en/project/GA17-00551S" target="_blank" >GA17-00551S: Relationship between butanol efflux and butanol tolerance of Clostridia</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
MicrobiologyOpen
ISSN
2045-8827
e-ISSN
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Volume of the periodical
neuveden
Issue of the periodical within the volume
DEC 2020
Country of publishing house
US - UNITED STATES
Number of pages
14
Pages from-to
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UT code for WoS article
000598372300001
EID of the result in the Scopus database
2-s2.0-85100952913