Mutagenic strategies against luxS gene affect the early stage of biofilm formation of Campylobacter jejuni

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F21%3A43923768" target="_blank" >RIV/60461373:22330/21:43923768 - isvavai.cz</a>

Result on the web

<a href="https://link.springer.com/article/10.1007/s13353-021-00655-y" target="_blank" >https://link.springer.com/article/10.1007/s13353-021-00655-y</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s13353-021-00655-y" target="_blank" >10.1007/s13353-021-00655-y</a>

Result language

angličtina

Original language name

Mutagenic strategies against luxS gene affect the early stage of biofilm formation of Campylobacter jejuni

Original language description

Currently, it is clear that the luxS gene has an impact on the process of biofilm formation in Campylobacter jejuni. However, even within the species, naturally occurring strains of Campylobacter lacking the luxS gene exist, which can form biofilms. In order to better understand the genetic determinants and the role of quorum sensing through the LuxS/AI-2 pathway in biofilm formation, a set of mutant/complemented strains of C. jejuni 81–176 were prepared. Additionally, the impact of the mutagenic strategy used against the luxS gene was investigated. Biofilm formation was affected by both the presence and absence of the luxS gene, and by the mutagenic strategy used. Analysis by CLSM showed that all mutant strains formed significantly less biofilm mass when compared to the wild-type. Interestingly, the deletion mutant (∆luxS) showed a larger decrease in biofilm mass than the substitution (∙luxS) and insertional inactivated (: : luxS) mutants, even though all the mutant strains lost the ability to produce autoinducer-2 molecules. Moreover, the biofilm of the ∆luxS mutant lacked the characteristic microcolonies observed in all other strains. The complementation of all mutant strains resulted in restored ability to produce AI-2, to form a complex biofilm, and to develop microcolonies at the level of the wild-type. © 2021, Institute of Plant Genetics, Polish Academy of Sciences, Poznan.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10606 - Microbiology

Project

<a href="/en/project/GA18-16549S" target="_blank" >GA18-16549S: The prevalence and diversity of Campylobacter jejuni isolates and identification of factors facilitating their survival</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2021

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Applied Genetics

ISSN

1234-1983

e-ISSN

—

Volume of the periodical

63

Issue of the periodical within the volume

1

Country of publishing house

DE - GERMANY

Number of pages

13

Pages from-to

145-157

UT code for WoS article

000689526800001

EID of the result in the Scopus database

2-s2.0-85113649391