Non-thermal plasma causes Pseudomonas aeruginosa biofilm release to planktonic form and inhibits production of Las-B elastase, protease and pyocyanin
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F22%3A43924561" target="_blank" >RIV/60461373:22330/22:43924561 - isvavai.cz</a>
Alternative codes found
RIV/60461373:22340/22:43924561 RIV/61388963:_____/22:00563284
Result on the web
<a href="https://www.frontiersin.org/articles/10.3389/fcimb.2022.993029/full" target="_blank" >https://www.frontiersin.org/articles/10.3389/fcimb.2022.993029/full</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3389/fcimb.2022.993029" target="_blank" >10.3389/fcimb.2022.993029</a>
Alternative languages
Result language
angličtina
Original language name
Non-thermal plasma causes Pseudomonas aeruginosa biofilm release to planktonic form and inhibits production of Las-B elastase, protease and pyocyanin
Original language description
The increasing risk of antibiotic failure in the treatment of Pseudomonas aeruginosa infections is largely related to the production of a wide range of virulence factors. The use of non-thermal plasma (NTP) is a promising alternative to antimicrobial treatment. Nevertheless, there is still a lack of knowledge about the effects of NTP on the virulence factors production. We evaluated the ability of four NTP-affected P. aeruginosa strains to re-form biofilm and produce Las-B elastase, proteases, lipases, haemolysins, gelatinase or pyocyanin. Highly strains-dependent inhibitory activity of NTP against extracellular virulence factors production was observed. Las-B elastase activity was reduced up to 82% after 15-min NTP treatment, protease activity and pyocyanin production by biofilm cells was completely inhibited after 60 min, in contrast to lipases and gelatinase production, which remained unchanged. However, for all strains tested, a notable reduction in biofilm re-development ability was depicted using spinning disc confocal microscopy. In addition, NTP exposure of mature biofilms caused disruption of biofilm cells and their dispersion into the environment, as shown by transmission electron microscopy. This appears to be a key step that could help overcome the high resistance of P. aeruginosa and its eventual elimination, for example in combination with antibiotics still highly effective against planktonic cells. Copyright © 2022 Kašparová, Vaňková, Paldrychová, Svobodová, Hadravová, Jarošová Kolouchová, Masák and Scholtz.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10606 - Microbiology
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2022
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Frontiers in Cellular and Infection Microbiology
ISSN
2235-2988
e-ISSN
2235-2988
Volume of the periodical
12
Issue of the periodical within the volume
SEP 23 2022
Country of publishing house
CH - SWITZERLAND
Number of pages
16
Pages from-to
nestrankovano
UT code for WoS article
000865023700001
EID of the result in the Scopus database
2-s2.0-85139408657