Chitosan isolated from black soldier flies Hermetia illucens: Structure and enzymatic hydrolysis
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F22%3A43925067" target="_blank" >RIV/60461373:22330/22:43925067 - isvavai.cz</a>
Result on the web
<a href="https://www.sciencedirect.com/science/article/pii/S1359511322001362" target="_blank" >https://www.sciencedirect.com/science/article/pii/S1359511322001362</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.procbio.2022.04.020" target="_blank" >10.1016/j.procbio.2022.04.020</a>
Alternative languages
Result language
angličtina
Original language name
Chitosan isolated from black soldier flies Hermetia illucens: Structure and enzymatic hydrolysis
Original language description
This study aims to elucidate the biochemical properties of chitosan isolated and purified from larvae of black soldier flies Hermetia illucens to discover and utilize new eco-friendly chitin material. The productivity of chitin obtained from the larvae of black soldier flies H. illucens was found to be approximately 5.00 +/- 0.002%. These chitins were converted to chitosan by chemical treatment with a yield of about 93 +/- 0.16%, and a deacetylation degree of 85.71 +/- 0.24% was confirmed. The average molecular weight of the purified chitosan designated to Hi-Cs was estimated to be approximately 680-kDa by high-performance gel permeation chromatography (HPGPC). The primary chemical structure of the Hi-Cs and its hydrolysates designated to active molecule chitosan (AMC) was identified by Fourier-transform infrared spectroscopy (FT-IR), H-1 Nuclear magnetic resonance (H-1 NMR), and Matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry analyses, respectively. The significant antioxidant activity of AMC was confirmed through the ABTS radical removal activity analysis. To the best of our knowledge, this is the first report on the structural analysis and antioxidant activity of chitosan isolated and purified from larvae of black soldier flies H. illucens.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2022
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Process Biochemistry
ISSN
0032-9592
e-ISSN
1873-3298
Volume of the periodical
118
Issue of the periodical within the volume
July
Country of publishing house
BE - BELGIUM
Number of pages
11
Pages from-to
171-181
UT code for WoS article
000860796900008
EID of the result in the Scopus database
2-s2.0-85129515262