Changes in the gut bacteriome upon gluten-free diet intervention do not mediate beta cell preservation

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F22%3A43925154" target="_blank" >RIV/60461373:22330/22:43925154 - isvavai.cz</a>

Alternative codes found

RIV/60460709:41210/23:96155 RIV/00216208:11130/23:10448592 RIV/00216208:11320/23:10448592 RIV/00216208:11120/23:43924036 and 2 more

Result on the web

<a href="https://link.springer.com/article/10.1007/s00125-022-05805-3" target="_blank" >https://link.springer.com/article/10.1007/s00125-022-05805-3</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s00125-022-05805-3" target="_blank" >10.1007/s00125-022-05805-3</a>

Result language

angličtina

Original language name

Changes in the gut bacteriome upon gluten-free diet intervention do not mediate beta cell preservation

Original language description

Aims/hypothesis: We previously detected indications that beta cell function is protected by gluten-free diet (GFD) introduced shortly after the onset of childhood type 1 diabetes. The present aim was to assess whether GFD was associated with changes in the gut bacteriome composition and in its functional capacity, and whether such changes mediated the observed effects of GFD on beta cell function. Methods: Forty-five children (aged 10.2 ± 3.3 years) were recruited into a self-selected intervention trial primarily focused on determining the role of GFD on beta cell preservation (ClinicalTrials.gov NCT02867436). Stool samples were collected prior to the dietary intervention and then at 3-month intervals. A total of 128 samples from the GFD group and 112 from the control group were analysed for bacteriome 16S rDNA community profiles, the bacteriome functional capacity was predicted using PICRUSt2 and actual gut metabolome profiles measured using NMR. Intestinal permeability was assessed using serum zonulin concentrations at 1, 6 and 12 months and lactulose/mannitol tests at the end of intervention. Dietary questionnaires were used to ensure that the dietary intervention did not result in differences in energy or nutrient intake. Results: The bacteriome community composition changed during the intervention with GFD: of abundant genera, a 3.3-fold decrease was noted for Bifidobacterium genus (adjusted p=1.4 × 10−4 in a DESeq2 model, p=0.026 in generalised estimating equations model), whereas a 2.4-fold increase was observed in Roseburia (adjusted p=0.02 in DESeq2 model, p=0.002 in generalised estimating equations model). The within-sample (alpha) diversity did not change, and there was no statistically significant clustering of GFD samples in the ordination graphs of beta diversity. Neither of the genera changes upon GFD intervention showed any association with the pace of beta cell loss (p&gt;0.50), but of the remaining taxa, several genera of Bacteroidaceae family yielded suggestive signals. The faecal metabolome profile ordination correlated with that of bacteriomes but did not associate with GFD or categories of beta cell preservation. There was no indication of changes in gut permeability. Conclusions/interpretation: The bacteriome reacted to GFD, but the changes were unrelated to the pace of beta cell capacity loss. The previously observed moderately protective effect of GFD is therefore mediated through other pathways. Graphical abstract: [Figure not available: see fulltext.] © 2022, The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

30209 - Paediatrics

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

V - Vyzkumna aktivita podporovana z jinych verejnych zdroju

Publication year

2022

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Diabetologia

ISSN

0012-186X

e-ISSN

1432-0428

Volume of the periodical

66

Issue of the periodical within the volume

leden 2023

Country of publishing house

DE - GERMANY

Number of pages

6

Pages from-to

241-246

UT code for WoS article

000864236500003

EID of the result in the Scopus database

2-s2.0-85139202797