Quantification of conjugated type A trichothecenes in cereals using immuno-affinity clean-up and enzymatic hydrolysis
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F22%3A43925452" target="_blank" >RIV/60461373:22330/22:43925452 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Quantification of conjugated type A trichothecenes in cereals using immuno-affinity clean-up and enzymatic hydrolysis
Original language description
Currently, increasing attention has been paid to conjugated mycotoxins in plant-based foods because of their assumed bioavailability and health risks. However, analytical standards for most mycotoxin glycosides are not available and risk assessment processes have not yet been completed because of the lack of occurrence data. Our study presents a method for efficient pre-concentration of type A trichothecene glycosides using immuno-affinity columns (IAC), followed by indirect determination after enzymatic hydrolysis. Cross-reactivity of antibodies of four types of commercially available IACs dedicated to HT-2/T-2 was tested for HT-2/T-2 glycosides. For enzymatic hydrolysis of β-glycosidic bond between HT-2/T-2 and releasing the saccharidic moiety, two different β- glycosidases were tested. Separation of free and glycosylated mycotoxins before and after hydrolysis was performed on Acquity UPLC® HSS T3 reversed phase column (100 mm x 2.1 mm, 1.8 mm; Waters), with subsequent detection assured by Q-orbitrap mass spectrometer in full-spectral acquisition mode with systematic fragmentation of precursor ions in ESI+ mode. This method was applied to 52 various oat-based products. Free T-2 toxin and T-2-monoglucosides were present in 92% and 69% of samples, respectively. Free HT-2 toxin and HT-2-monoglucosides were present in 92% of samples and HT-2-diglucosides were detected in 38% of samples. The percentage of HT-2 present in the form of glycosides was up to 56% of the total HT-2 content.
Czech name
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Czech description
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Classification
Type
O - Miscellaneous
CEP classification
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OECD FORD branch
10406 - Analytical chemistry
Result continuities
Project
<a href="/en/project/LM2018100" target="_blank" >LM2018100: Infrastructure for Promoting Metrology in Food and Nutrition in the Czech Republic</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach
Others
Publication year
2022
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů