Critical Assessment of Clean-up Procedures Used for Gas Chromatography-Mass Spectrometry Analysis of Pesticide Residues in Cannabis Inflorescence

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F24%3A43930186" target="_blank" >RIV/60461373:22330/24:43930186 - isvavai.cz</a>

Result on the web

<a href="https://pubmed.ncbi.nlm.nih.gov/39600141/" target="_blank" >https://pubmed.ncbi.nlm.nih.gov/39600141/</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/jssc.70033" target="_blank" >10.1002/jssc.70033</a>

Result language

angličtina

Original language name

Critical Assessment of Clean-up Procedures Used for Gas Chromatography-Mass Spectrometry Analysis of Pesticide Residues in Cannabis Inflorescence

Original language description

Accurate analysis of pesticide residues in such a complex matrix as cannabis is a challenging task. The aim of this study was to find an optimal way of removing abundant matrix co-extracts from Quick, Easy, Cheap, Effective, Rugged, and Safe extract prior to its analysis by gas chromatography-tandem mass spectrometry (GC-MS/MS). Out of the seven procedures tested, clean-up with Supel QuE Verde sorbent provided the most satisfactory performance characteristics for 277 targeted pesticides, which is the highest number of these analytes ever investigated within a GC-MS-based study focused on cannabis. The criteria set in the SANTE/11312/2021 V2 document were considered to assess the results obtained within the method validation. Recoveries in the range of 70%–120% with repeatability ≤20% were obtained for 219 and 114 pesticides at spiking levels of 0.1 and 0.01 mg/kg, respectively. For a deeper insight into the function of tested sorbents and to understand the effects of interfering matrix co-extracts, the two-dimensional GC coupled with the time-of-flight MS method was employed for the analysis of all purified extracts. Co-eluting major phytocannabinoids and free fatty acids were shown to be responsible for high “chemical noise”, increasing limits of quantification of pesticides at respective retention times. Since cannabis chemotypes fairly differ in phytocannabinoid content, the matrix effects, thus performance characteristics, may vary. Under such conditions, validation is recommended for different cannabis chemotypes. © 2024 Wiley-VCH GmbH.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10406 - Analytical chemistry

Project

<a href="/en/project/LM2023064" target="_blank" >LM2023064: Infrastructure for Promoting Metrology in Food and Nutrition in the Czech Republic</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Publication year

2024

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

JOURNAL OF SEPARATION SCIENCE

ISSN

1615-9306

e-ISSN

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Volume of the periodical

47

Issue of the periodical within the volume

22

Country of publishing house

TW - TAIWAN (PROVINCE OF CHINA)

Number of pages

11

Pages from-to

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UT code for WoS article

001369932600001

EID of the result in the Scopus database

2-s2.0-85210497400