Determination of seventeen phytocannabinoids in various matrices by UHPLC-HRMS/MS

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F20%3A43920883" target="_blank" >RIV/60461373:22330/20:43920883 - isvavai.cz</a>

Result on the web

<a href="https://www.chromatographyonline.com/view/determination-seventeen-phytocannabinoids-various-matrices-uhplc-hrmsms" target="_blank" >https://www.chromatographyonline.com/view/determination-seventeen-phytocannabinoids-various-matrices-uhplc-hrmsms</a>

DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Determination of seventeen phytocannabinoids in various matrices by UHPLC-HRMS/MS

Original language description

A simple ultrahigh-performance liquid chromatography high resolution tandem mass spectrometry (UHPLC− HRMS/MS) method to determine seventeen phytocannabinoids was developed and validated for Cannabis plants, resins and their extracts, and oils. The analysis was challenging because of the complexity of matrices, the large differences in the concentration of phytocannabinoids and their pattern in various cultivars, and the structural similarity of these analytes. Method performance characteristics were obtained using internal reference materials. The analytes were isolated from dry hemp by repeated extraction using ethanol with recoveries in the range of 95–100%. For the resinous matrices and oils, samples were directly dissolved in ethanol. Repeatability was 2–12% for all analyte–matrix combinations. Limits of quantification (LOQs) were within the range of 0.5–1.0 mg/kg for dry hemp, 2.5–5.0 mg/kg for resins, and 0.25–0.50 mg/kg for oils. As a result of high matrix dilution, calibration standards in net solvent could be used for phytocannabinoids quantification because matrix effects were almost eliminated. Nevertheless, to ensure the trueness of generated data regardless of the matrix tested, internal standards of delta‑9‑tetrahydrocannabinol-D3 (Δ9-THC-D3), cannabidiol-D3 (CBD-D3) and cannabinol-D3 (CBN-D3) were also used.

Czech name

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Czech description

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Type

J_ost - Miscellaneous article in a specialist periodical

CEP classification

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OECD FORD branch

10406 - Analytical chemistry

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2020

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

LC GC Europe

ISSN

1471-6577

e-ISSN

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Volume of the periodical

33

Issue of the periodical within the volume

1

Country of publishing house

US - UNITED STATES

Number of pages

9

Pages from-to

8-16

UT code for WoS article

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EID of the result in the Scopus database

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